Department of Chemistry, Faculty of Science and Letter, Kafkas University, Kars, Turkey.
Department of Chemistry and Chemical Processing Technologies Kars Vocational School, Kafkas University, Kars, Turkey.
Biol Trace Elem Res. 2023 Sep;201(9):4429-4436. doi: 10.1007/s12011-022-03503-0. Epub 2022 Dec 2.
Aluminum (Al) is one of the most abundant element in the world. But aluminum exposure and accumulation causes serious diseases, related with free radicals. Reduced glutathione (GSH) is a tripeptide with intracellular antioxidant effects. This study aimed to investigate the role of GSH on adenosine deaminase (ADA), antioxidant system, and aluminum and zinc (Zn) levels in acute aluminum toxicity. In this study, Sprague-Dawley rats (n = 32) were used. The rats were divided into four equal groups (n = 8). Group I received 0.5 mL intraperitoneal injection of 0.9% saline solution (NaCI), Group II received single-dose AlCI, Group III was given GSH for seven days, and Group IV was given AlCI single dose, and at the same time, 100 mg/kg GSH was given for seven days. At the end of the trial, blood samples were collected by cardiac puncture. Serum total antioxidant status (TAS) and Zn levels were lower in the aluminum-administered group than the control group. In contrast, plasma total oxidant status (TOS) and aluminum concentrations and ADA activity were found higher in the aluminum-administered group than in the control group. Unlike the other groups, group GSH administrated with aluminum was similar to the control group. As a result, GSH administration has a regulatory effect on ADA activity, antioxidant system, and Zn levels in experimental aluminum toxicity. In addition, GSH may reduce the oxidant capacity increased by Al administration and may have a tolerant role on the accumulated serum Al levels. But long-term experimental Al toxicity studies are needed to reach a firm conclusion.
铝（Al）是世界上最丰富的元素之一。但是，铝暴露和积累会导致与自由基有关的严重疾病。还原型谷胱甘肽（GSH）是一种具有细胞内抗氧化作用的三肽。本研究旨在探讨 GSH 对急性铝毒性中腺苷脱氨酶（ADA）、抗氧化系统以及铝和锌（Zn）水平的作用。本研究使用了 Sprague-Dawley 大鼠（n=32）。将大鼠分为四组（n=8）。第 I 组接受 0.5 mL 腹腔注射 0.9%生理盐水（NaCI），第 II 组接受单次剂量 AlCI，第 III 组给予 GSH 七天，第 IV 组给予单次剂量 AlCI，同时给予 100 mg/kg GSH 七天。试验结束时，通过心脏穿刺采集血样。与对照组相比，铝处理组的血清总抗氧化状态（TAS）和 Zn 水平较低。相反，与对照组相比，铝处理组的血浆总氧化状态（TOS）和铝浓度以及 ADA 活性更高。与其他组不同的是，给予 GSH 和铝的 GSH 组与对照组相似。因此，GSH 给药对实验性铝毒性中的 ADA 活性、抗氧化系统和 Zn 水平具有调节作用。此外，GSH 可能降低铝给药引起的氧化能力，并可能对蓄积的血清 Al 水平具有耐受作用。但是，需要进行长期的实验性铝毒性研究才能得出确定的结论。
