Geoghegan C M, Houghton J A
Department of Bacteriology, Agricultural Institute, Cork, Ireland.
J Gen Microbiol. 1987 Jan;133(1):119-26. doi: 10.1099/00221287-133-1-119.
The unicellular cyanobacterium Gloeocapsa alpicola contains both photoreactivation and excision repair mechanisms for correcting UV-induced damage to its cellular DNA. An 11.5 kb EcoRI fragment was isolated from a cosmid bank of G. alpicola and was shown to complement a recA deletion in Escherichia coli S.17 and JC10289. These recA strains showed increased survival to UV and methyl methanesulphonate (MMS) when transformed with the cyanobacterial DNA fragment, and also showed filamentation in response to UV irradiation. Preliminary analysis of the protein encoded by the cyanobacterial DNA fragment indicated a major protein of 39,000 Da; this is very similar in size to the recA protein of E. coli.
单细胞蓝藻高山聚球藻含有光复活和切除修复机制，用于纠正紫外线对其细胞DNA造成的损伤。从高山聚球藻的黏粒文库中分离出一个11.5 kb的EcoRI片段，该片段被证明可弥补大肠杆菌S.17和JC10289中的recA缺失。当用该蓝藻DNA片段转化时，这些recA菌株对紫外线和甲磺酸甲酯（MMS）的存活率增加，并且在紫外线照射下也会出现丝状化。对该蓝藻DNA片段编码的蛋白质的初步分析表明，有一个主要的39,000 Da蛋白质；其大小与大肠杆菌的recA蛋白非常相似。
