Shinkawa H, Sugiyama M, Nimi O
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Higashi-Hiroshima, Japan.
J Gen Microbiol. 1987 May;133(5):1289-96. doi: 10.1099/00221287-133-5-1289.
The nucleotide sequence of the DNA fragment containing the streptomycin phosphotransferase (streptomycin kinase) [corrected] gene from the streptomycin-producer Streptomyces griseus strain HUT 6037 was determined. Analysis of the sequence revealed an open reading frame which could encode 325 amino acid residues. A biased codon usage pattern, reflecting the high G + C composition (approximately 74%) of Streptomyces DNA, was observed in the gene.
测定了来自链霉素产生菌灰色链霉菌HUT 6037菌株、含有链霉素磷酸转移酶(链霉素激酶)[校正后]基因的DNA片段的核苷酸序列。对该序列的分析揭示了一个可编码325个氨基酸残基的开放阅读框。在该基因中观察到一种偏向性的密码子使用模式,这反映了链霉菌DNA的高G + C组成(约74%)。