Lee Hoyoon, Lee Kisung, Lee Byoung-Kwon, Priezzhev Alexander V, Shin Sehyun
School of Mechanical Engineering, Korea University, Seoul, Korea.
Department of Experimental Physics, University of Saarland, Saarbrücken, Germany.
Clin Hemorheol Microcirc. 2017;66(2):97-104. doi: 10.3233/CH-16191.
Mechanical shear stress is one of the important factors for platelet activation. Although shear stress has been frequently utilized in many applications of diagnostic bio-equipment, there has been little consideration as to whether shear stress induces platelet activation and consequently alters hemorheological characteristics. Therefore, we investigated the effect of shear-induced platelet activation on red blood cell (RBC) aggregation. The hypothesis of the present research is as follows: Platelets activated by high shear stress secrete substances, which can affect hemorheological characteristics to promote RBC aggregation. In our study, an increase in RBC aggregation indices (critical shear stress (CSS) and aggregation index (AI)) by shear-induced platelet activation was observed. Significantly, an increase of 19% in CSS was observed. However, deformability remained unchanged. These phenomena could be a result of the increased cellular adhesion force on RBC membranes due to secreted substances from activated platelets. Therefore, since high shear application results in the unexpected effect on RBC aggregation, conditions for shear application in diagnostic bio-equipment are to be carefully determined.
机械剪切应力是血小板活化的重要因素之一。尽管剪切应力已在诊断生物设备的许多应用中频繁使用，但对于剪切应力是否会诱导血小板活化并因此改变血液流变学特性却很少有人考虑。因此，我们研究了剪切诱导的血小板活化对红细胞（RBC）聚集的影响。本研究的假设如下：高剪切应力激活的血小板会分泌物质，这些物质会影响血液流变学特性以促进红细胞聚集。在我们的研究中，观察到剪切诱导的血小板活化使红细胞聚集指数（临界剪切应力（CSS）和聚集指数（AI））增加。值得注意的是，CSS 增加了 19%。然而，变形性保持不变。这些现象可能是由于活化血小板分泌的物质导致红细胞膜上细胞粘附力增加的结果。因此，由于高剪切作用会对红细胞聚集产生意想不到的影响，所以在诊断生物设备中应用剪切的条件需要仔细确定。
