Sala Priscila, Torrinhas Raquel Susana, Fonseca Danielle Cristina, Heymsfield Steven, Giannella-Neto Daniel, Waitzberg Dan Linetzky
FMUSP-Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, University of São Paulo (LIM 35), São Paulo, Brazil.
Pennington Biomedical Research Center, Baton Rouge, LA, USA.
Obes Surg. 2017 Apr;27(4):1123-1127. doi: 10.1007/s11695-017-2602-0.
Intestinal expression of regenerating pancreatic islet-derived protein-encoding genes (REG) would be enhanced after Roux-en-Y gastric bypass (RYGB) and would affect postoperative type 2 diabetes remission (T2Dr).
Intestinal biopsy samples were collected from 20 adult obese women with T2D before and 3 months after RYGB. Levels of REG expression and the gene encoding its putative receptor (EXTL3) were assessed by microarray and validated by quantitative RT-PCR. T2Dr was assessed according to ADA criteria 1 year after RYGB.
After RYGB, only patients with T2Dr had significantly increased REG1α and REG3γ expression in the jejunum, as validated by quantitative RT-PCR.
Our data provide support for the hypothesis that increased jejunal expression of REG genes after RYGB affects T2Dr, possibly by playing an endocrine function.
在Roux-en-Y胃旁路术（RYGB）后，再生胰岛衍生蛋白编码基因（REG）的肠道表达会增强，并会影响术后2型糖尿病缓解（T2Dr）。
收集20名成年肥胖2型糖尿病女性患者在RYGB术前及术后3个月的肠道活检样本。通过微阵列评估REG表达水平及其假定受体（EXTL3）的编码基因，并通过定量逆转录聚合酶链反应进行验证。根据ADA标准在RYGB术后1年评估T2Dr。
RYGB术后，经定量逆转录聚合酶链反应验证，仅T2Dr患者空肠中REG1α和REG3γ表达显著增加。
我们的数据支持以下假设，即RYGB术后空肠中REG基因表达增加可能通过发挥内分泌功能影响T2Dr。
