Roux-en-Y胃旁路术后2型糖尿病缓解：空肠中编码再生胰岛衍生蛋白的基因表达增加作为潜在机制的证据
Type 2 Diabetes Remission After Roux-en-Y Gastric Bypass: Evidence for Increased Expression of Jejunal Genes Encoding Regenerating Pancreatic Islet-Derived Proteins as a Potential Mechanism.
作者信息
Sala Priscila, Torrinhas Raquel Susana, Fonseca Danielle Cristina, Heymsfield Steven, Giannella-Neto Daniel, Waitzberg Dan Linetzky
机构信息
FMUSP-Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, University of São Paulo (LIM 35), São Paulo, Brazil.
Pennington Biomedical Research Center, Baton Rouge, LA, USA.
出版信息
Obes Surg. 2017 Apr;27(4):1123-1127. doi: 10.1007/s11695-017-2602-0.
BACKGROUND
Intestinal expression of regenerating pancreatic islet-derived protein-encoding genes (REG) would be enhanced after Roux-en-Y gastric bypass (RYGB) and would affect postoperative type 2 diabetes remission (T2Dr).
METHODS
Intestinal biopsy samples were collected from 20 adult obese women with T2D before and 3 months after RYGB. Levels of REG expression and the gene encoding its putative receptor (EXTL3) were assessed by microarray and validated by quantitative RT-PCR. T2Dr was assessed according to ADA criteria 1 year after RYGB.
RESULTS
After RYGB, only patients with T2Dr had significantly increased REG1α and REG3γ expression in the jejunum, as validated by quantitative RT-PCR.
CONCLUSIONS
Our data provide support for the hypothesis that increased jejunal expression of REG genes after RYGB affects T2Dr, possibly by playing an endocrine function.
背景
在Roux-en-Y胃旁路术（RYGB）后，再生胰岛衍生蛋白编码基因（REG）的肠道表达会增强，并会影响术后2型糖尿病缓解（T2Dr）。
方法
收集20名成年肥胖2型糖尿病女性患者在RYGB术前及术后3个月的肠道活检样本。通过微阵列评估REG表达水平及其假定受体（EXTL3）的编码基因，并通过定量逆转录聚合酶链反应进行验证。根据ADA标准在RYGB术后1年评估T2Dr。
结果
RYGB术后，经定量逆转录聚合酶链反应验证，仅T2Dr患者空肠中REG1α和REG3γ表达显著增加。
结论
我们的数据支持以下假设，即RYGB术后空肠中REG基因表达增加可能通过发挥内分泌功能影响T2Dr。
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