巴基斯坦2型糖尿病患者中REG Iα基因的表达

Expression of REG Iα gene in type 2 diabetics in Pakistan.

作者信息

Uppal Sadaf Saleem, Naveed Abdul Khaliq, Baig Saeeda, Chaudhry Bushra

机构信息

Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi and National University of Science and Technology, Islamabad, Pakistan.

Department of Biochemistry, Islamic International Medical College, Riphah International University, Islamabad, Pakistan.

出版信息

Diabetol Metab Syndr. 2015 Nov 13;7:96. doi: 10.1186/s13098-015-0092-6. eCollection 2015.

DOI:10.1186/s13098-015-0092-6

PMID:26568772

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4643495/

Abstract

BACKGROUND

The escalating rate of diabetes' has prompted researchers around the world to explore for early markers. A deficit of functional β-cell mass plays a central role in the pathophysiology of type 2 diabetes. The REG (Regenerating) gene, encoding a 166 amino acid REG protein was discovered in rats and humans which is released in response to β-cells damage and play a role in their regeneration. The objective of this study was to characterize serum levels of REG Iα proteins in type 2 diabetic patients as indicator of β-cell apoptosis as well as regeneration.

METHODS

Unrelated type 2 diabetic patients (n = 55) of different age groups and disease duration were recruited from the Medical OPD of PNS Shifa Hospital. Age and sex matched non diabetic controls (n = 20) without family history of diabetes were selected from the same setting. Demographical details were recorded on a structured questionnaire. Biochemical parameters like FBG, HbA1c, TC and TG levels were measured. Serum levels of REG Iα protein were determined by ELISA.

RESULTS

Levels of REG Iα protein were found significantly raised in type 2 diabetic patients compared to controls (p < 001). Patients with short duration of the disease had higher levels of REG Iα as compared to patients with longer duration of the disease. Although the patients were on anti hyperglycemic agents, a positive correlation was found between REG Iα serum levels, FBG and HbA1c levels. Patients with higher BMI had higher levels of serum REG Iα levels. Serum TC, TG and Hb levels showed no correlation.

CONCLUSION

REG Iα may be used as a marker/predictor of type 2 diabetes especially in the early stages of the disease.

摘要

背景

糖尿病发病率的不断上升促使世界各地的研究人员探索早期标志物。功能性β细胞量的不足在2型糖尿病的病理生理学中起着核心作用。在大鼠和人类中发现了编码166个氨基酸的REG蛋白的REG（再生）基因，该基因在β细胞受损时释放，并在其再生中发挥作用。本研究的目的是将2型糖尿病患者血清中REG Iα蛋白水平作为β细胞凋亡及再生的指标进行特征分析。

方法

从PNS Shifa医院内科门诊招募不同年龄组和病程的非亲属2型糖尿病患者（n = 55）。从同一环境中选取年龄和性别匹配、无糖尿病家族史的非糖尿病对照者（n = 20）。通过结构化问卷记录人口统计学细节。测量空腹血糖（FBG）、糖化血红蛋白（HbA1c）、总胆固醇（TC）和甘油三酯（TG）等生化参数。采用酶联免疫吸附测定法（ELISA）测定血清REG Iα蛋白水平。

结果

与对照组相比，2型糖尿病患者的REG Iα蛋白水平显著升高（p < 0.01）。病程短的患者的REG Iα水平高于病程长的患者。尽管患者正在服用抗高血糖药物，但REG Iα血清水平与FBG和HbA1c水平之间存在正相关。体重指数（BMI）较高的患者血清REG Iα水平较高。血清TC、TG和血红蛋白水平无相关性。