Kumar Ravi Bhushan, Alam Syed Imteyaz
Biotechnology Division, Defence Research and Development Establishment, Gwalior, 474002, India.
Folia Microbiol (Praha). 2017 Jul;62(4):343-353. doi: 10.1007/s12223-017-0503-1. Epub 2017 Feb 17.
Clostridium perfringens is a Validated Biological Agent and a pathogen of medical, veterinary, and military significance. Gas gangrene is the most destructive of all the clostridial diseases and is caused by C. perfringens type A strains wherein the infection spreads quickly (several inches per hour) with production of gas. Influence of repeated in vitro cultivation on the infectivity of C. perfringens was investigated by comparing the surface proteins of laboratory strain and repository strains of the bacterium using 2DE-MS approach. In order to optimize host-pathogen interaction during experimental gas gangrene infection, we also explored the role of particulate matrix on ability of C. perfringens to cause gas gangrene.
产气荚膜梭菌是一种经过验证的生物制剂，也是具有医学、兽医和军事意义的病原体。气性坏疽是所有梭菌性疾病中破坏性最大的，由A型产气荚膜梭菌菌株引起，感染会迅速扩散（每小时数英寸）并产生气体。通过使用二维电泳-质谱方法比较该细菌实验室菌株和保藏菌株的表面蛋白，研究了反复体外培养对产气荚膜梭菌感染性的影响。为了在实验性气性坏疽感染期间优化宿主-病原体相互作用，我们还探讨了颗粒基质对产气荚膜梭菌引起气性坏疽能力的作用。
