Varsani Arvind, Roumagnac Philippe, Fuchs Marc, Navas-Castillo Jesús, Moriones Enrique, Idris Ali, Briddon Rob W, Rivera-Bustamante Rafael, Murilo Zerbini F, Martin Darren P
The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine and School of Life Sciences, Arizona State University, Tempe, 85287-5001, AZ, USA.
Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Cape Town, 7701, South Africa.
Arch Virol. 2017 Jun;162(6):1819-1831. doi: 10.1007/s00705-017-3268-6. Epub 2017 Feb 17.
Geminiviruses are plant-infecting single-stranded DNA viruses that occur in most parts of the world. Currently, there are seven genera within the family Geminiviridae (Becurtovirus, Begomovirus, Curtovirus, Eragrovirus, Mastrevirus, Topocuvirus and Turncurtovirus). The rate of discovery of new geminiviruses has increased significantly over the last decade as a result of new molecular tools and approaches (rolling-circle amplification and deep sequencing) that allow for high-throughput workflows. Here, we report the establishment of two new genera: Capulavirus, with four new species (Alfalfa leaf curl virus, Euphorbia caput-medusae latent virus, French bean severe leaf curl virus and Plantago lanceolata latent virus), and Grablovirus, with one new species (Grapevine red blotch virus). The aphid species Aphis craccivora has been shown to be a vector for Alfalfa leaf curl virus, and the treehopper species Spissistilus festinus is the likely vector of Grapevine red blotch virus. In addition, two highly divergent groups of viruses found infecting citrus and mulberry plants have been assigned to the new species Citrus chlorotic dwarf associated virus and Mulberry mosaic dwarf associated virus, respectively. These species have been left unassigned to a genus by the ICTV because their particle morphology and insect vectors are unknown.
双生病毒是感染植物的单链DNA病毒，在世界大部分地区都有出现。目前，双生病毒科中有七个属（贝库托病毒属、菜豆金色花叶病毒属、曲顶病毒属、画眉草病毒属、玉米线条病毒属、番茄伪曲顶病毒属和芜菁曲顶病毒属）。由于新的分子工具和方法（滚环扩增和深度测序）允许高通量工作流程，在过去十年中，新双生病毒的发现率显著提高。在这里，我们报告建立了两个新属：卡普拉病毒属，包含四个新种（苜蓿卷叶病毒、美杜莎大戟潜伏病毒、法国菜豆严重卷叶病毒和窄叶车前潜伏病毒），以及格拉布病毒属，包含一个新种（葡萄红环斑病毒）。已证明蚜虫物种豆蚜是苜蓿卷叶病毒的传播媒介，而角顶叶蝉物种斯氏角顶叶蝉可能是葡萄红环斑病毒的传播媒介。此外，发现分别感染柑橘和桑树的两组高度不同的病毒已被分别归类为新种柑橘褪绿矮化相关病毒和桑花叶矮化相关病毒。国际病毒分类委员会尚未将这些物种归类到属，因为它们的粒子形态和昆虫传播媒介尚不清楚。
