Fang Yufeng, Tyler Brett M
Interdisciplinary Ph.D. Program in Genetics, Bioinformatics & Computational Biology, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA, 24061, USA.
Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA.
Mol Microbiol. 2017 May;104(4):621-635. doi: 10.1111/mmi.13652. Epub 2017 Mar 16.
Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that the oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into the nucleus that differ from conventional well-characterized NLSs from mammals and yeast. Here, we have characterized in depth the NLSs of a P. sojae basic leucine zipper transcription factor, PsbZIP1. Nuclear localization of PsbZIP1 was determined by a central conserved region overlapping the DNA binding domain. Mutational analysis of this region identified four distinct elements that contributed multiplicatively to nuclear localization, but the conserved DNA binding residues were not required. Three of the elements showed autonomous NLS activity and the fourth served as a nuclear localization enhancer. Sequences within two of the nuclear localization elements defined a new form of bipartite NLS consisting of a triplet of basic residues followed by a tail of scattered basic amino acids.
卵菌纲是不等鞭毛菌门中类似真菌的真核微生物。我们最近发现，卵菌纲植物病原菌大豆疫霉利用核定位信号（NLSs）将蛋白质转运到细胞核中，这些核定位信号与来自哺乳动物和酵母的传统且特征明确的核定位信号不同。在此，我们深入研究了大豆疫霉碱性亮氨酸拉链转录因子PsbZIP1的核定位信号。PsbZIP1的核定位由与DNA结合结构域重叠的中央保守区域决定。对该区域的突变分析确定了四个不同的元件，它们对核定位有累加作用，但保守的DNA结合残基并非必需。其中三个元件表现出自主核定位信号活性，第四个元件作为核定位增强子。两个核定位元件中的序列定义了一种新的二分核定位信号形式，由一组三个碱性残基后跟一串分散的碱性氨基酸组成。
