University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Strasse 24-25, Haus 20, 14476 Potsdam-Golm, Germany.
J Plant Physiol. 2010 May 1;167(7):583-6. doi: 10.1016/j.jplph.2009.11.016. Epub 2010 Jan 29.
Large molecules require a nuclear localization signal (NLS) for translocation into the nucleus. Classical NLSs are rich in basic amino acids and they represent three groups, based on their structural features: SV40 T-antigen-type, yeast mating factor Matalpha-2-type, and bipartite NLSs. DNA-binding-with-one-finger (DOF) transcription factors play important roles in plants, and although their nuclear localization has been demonstrated in several cases, public protein localization prediction tools fail to detect NLS motifs in these proteins. Here, we demonstrate that an atypical bipartite NLS with a 17 amino acid long linker between its flanking basic regions directs Arabidopsis thaliana DOF proteins to the cell nucleus. The novel bipartite NLS is highly conserved in plant DOF transcription factors, including the single DOF protein in the green alga Chlamydomonas reinhardtii.
大分子需要核定位信号 (NLS) 才能进入细胞核。经典的 NLS 富含碱性氨基酸，根据其结构特征可分为三组：SV40 T 抗原型、酵母交配因子 Matalpha-2 型和双部分 NLS。DNA 结合单指（DOF）转录因子在植物中发挥着重要作用，尽管已经在几种情况下证明了它们的核定位，但公共的蛋白质定位预测工具未能在这些蛋白质中检测到 NLS 基序。在这里，我们证明了一种由侧翼碱性区域之间的 17 个氨基酸长接头组成的非典型双部分 NLS，可将拟南芥的 DOF 蛋白引导至细胞核。该新的双部分 NLS 在植物 DOF 转录因子中高度保守，包括绿藻衣藻中的单个 DOF 蛋白。
