Vognsen Mikael, Fabian-Jessing Bjørn K, Secher Niels, Løfgren Bo, Dezfulian Cameron, Andersen Lars W, Granfeldt Asger
Research Center for Emergency Medicine, Aarhus University Hospital, Aarhus, Denmark.
Department of Anaesthesiology and Intensive Care Medicine, Aarhus University Hospital, Aarhus, Denmark.
Resuscitation. 2017 Apr;113:115-123. doi: 10.1016/j.resuscitation.2017.01.024. Epub 2017 Feb 15.
Animal models are widely used in cardiac arrest research. This systematic review aimed to provide an overview of contemporary animal models of cardiac arrest.
Using a comprehensive research strategy, we searched PubMed and EMBASE from March 8, 2011 to March 8, 2016 for cardiac arrest animal models. Two investigators reviewed titles and abstracts for full text inclusion from which data were extracted according to pre-defined definitions.
Search criteria yielded 1741 unique titles and abstracts of which 490 full articles were included. The most common animals used were pigs (52%) followed by rats (35%) and mice (6%). Studies favored males (52%) over females (16%); 17% of studies included both sexes, while 14% omitted to report on sex. The most common methods for induction of cardiac arrest were either electrically-induced ventricular fibrillation (54%), asphyxia (25%), or potassium (8%). The median no-flow time was 8min (quartiles: 5, 8, range: 0-37min). The majority of studies used adrenaline during resuscitation (64%), while bicarbonate (17%), vasopressin (8%) and other drugs were used less prevalently. In 53% of the studies, the post-cardiac arrest observation time was ≥24h. Neurological function was an outcome in 48% of studies while 43% included assessment of a cardiac outcome.
Multiple animal models of cardiac arrest exist. The great heterogeneity of these models along with great variability in definitions and reporting make comparisons between studies difficult. There is a need for standardization of animal cardiac arrest research and reporting.
动物模型在心脏骤停研究中被广泛应用。本系统评价旨在概述当代心脏骤停动物模型。
采用全面的研究策略，我们在2011年3月8日至2016年3月8日期间检索了PubMed和EMBASE数据库，以查找心脏骤停动物模型。两名研究人员对标题和摘要进行审查，以确定是否纳入全文，然后根据预先定义的定义提取数据。
检索标准产生了1741个独特的标题和摘要，其中490篇全文被纳入。使用最多的动物是猪（52%），其次是大鼠（35%）和小鼠（6%）。研究中雄性（52%）多于雌性（16%）；17%的研究纳入了两性，而14%的研究未报告性别。诱导心脏骤停最常用的方法是电诱导室颤（54%）、窒息（25%）或钾盐（8%）。无血流时间中位数为8分钟（四分位数：5，8，范围：0 - 37分钟）。大多数研究在复苏期间使用肾上腺素（64%），而碳酸氢盐（17%）、血管加压素（8%）和其他药物的使用较少。在53%的研究中，心脏骤停后的观察时间≥24小时。48%的研究将神经功能作为一项结果，而43%的研究包括对心脏结果的评估。
存在多种心脏骤停动物模型。这些模型的巨大异质性以及定义和报告方面的巨大差异使得研究之间的比较变得困难。需要对动物心脏骤停研究和报告进行标准化。
