Rauzi M
Université Côte d'Azur, CNRS, INSERM, iBV, Nice, France.
Methods Cell Biol. 2017;139:153-165. doi: 10.1016/bs.mcb.2016.11.003. Epub 2016 Dec 23.
Tissue morphogenesis is governed by mechanical forces generated by cell cytoskeletal networks. It has been shown that subcellular forces are responsible for cell shape changes. Nevertheless cells in a developing organism do not act in isolation: cells contact and adhere one another, and forces are transmitted from cell-to-cell throughout tissues. Understanding how forces are integrated at the tissue level and finally at the full animal scale is nowadays a major challenge that will allow shedding new light on how embryo morphogenesis takes place. In this chapter, I present a new laser-based technique to probe tissue coupling in a living Drosophila embryo. Such technique allows generating mechanical fix boundaries that can eventually impair or modulate cell flows and tissue displacements to probe tissue interaction.
组织形态发生受细胞细胞骨架网络产生的机械力控制。已表明亚细胞力负责细胞形状的变化。然而，发育中的生物体中的细胞并非孤立行动：细胞相互接触并黏附，力在整个组织中从一个细胞传递到另一个细胞。了解力如何在组织水平乃至整个动物尺度上整合，是当今一项重大挑战，这将有助于揭示胚胎形态发生的过程。在本章中，我介绍一种基于激光的新技术，用于探测活体果蝇胚胎中的组织耦合。这种技术能够产生机械固定边界，最终可能会损害或调节细胞流动和组织位移，以探测组织间的相互作用。
