Pazinato Fernanda M, Curcio Bruna da Rosa, Fernandes Cristina G, Santos Carlos A, Feijó Lorena S, Varela Antonio Sérgio, Nogueira Carlos E W
Department of Veterinary Clinics, College of Veterinary, Federal University of Pelotas - UFPel, RS, Brazil.
Department of Veterinary Clinics, College of Veterinary, Federal University of Pelotas - UFPel, RS, Brazil.
Theriogenology. 2017 Mar 15;91:77-81. doi: 10.1016/j.theriogenology.2016.12.009. Epub 2016 Dec 10.
The objective of this study was to assess the placental vasculature and microcotyledons in pregnant mares with chronic laminitis. Twenty-six pregnant mares were enrolled in the study, 13 had chronic laminitis (Laminitis Group) and 13 were healthy mares (Healthy Group). Arterial systolic pressure and heart rate were measured in the last 30 days of gestation. After foaling, the fetal membranes were grossly evaluated and samples were harvested for histopathologic examination. All mares had digitalized images taken from chorioallantois for histomorphometry analyses (software-NIH ImageJ). Images were assessed for: (i) arterioles from the allantoic region: total and lumen vascular diameter and vascular wall thickness; (ii) microcotiledonary and capillary area/field. Mares in the Laminitis Group showed hypertension, shorter gestational length, lower placental weight and lower birthweight (p < 0.05) foal in comparison with mares in the Healthy Group. Laminitis mares had a reduction of vascular lumen diameters in the uterine body and pregnant horn (p < 0.05), vascular wall thickening in the pregnant horn (p < 0.05) and smaller capillary area/field in the microcotyledons of uterine body and pregnant horn (p < 0.05). In conclusion, pregnant mares with chronic laminitis presented signs of hypertension syndrome, and vascular abnormalitities in placental vessels such as reduction in the vascular lumen and capillary area in the microcotyledones, and thickening of the vascular wall. Foals born from mares with chronic laminitis showed lower birth weight and shorter gestation lengths.
本研究的目的是评估患慢性蹄叶炎的妊娠母马的胎盘血管系统和微叶。26匹妊娠母马被纳入研究，其中13匹患有慢性蹄叶炎（蹄叶炎组），13匹为健康母马（健康组）。在妊娠的最后30天测量动脉收缩压和心率。分娩后，对胎膜进行大体评估，并采集样本进行组织病理学检查。所有母马均采集了来自尿囊绒毛膜的数字化图像用于组织形态计量学分析（软件 - NIH ImageJ）。对图像评估以下内容：(i) 尿囊区域的小动脉：血管总直径、管腔直径和血管壁厚度；(ii) 微叶和毛细血管面积/视野。与健康组母马相比，蹄叶炎组母马表现出高血压、妊娠期较短、胎盘重量较低和驹出生体重较低（p < 0.05）。蹄叶炎母马子宫体和妊娠角的血管管腔直径减小（p < 0.05），妊娠角的血管壁增厚（p < 0.05），子宫体和妊娠角微叶中的毛细血管面积/视野较小（p < 0.05）。总之，患慢性蹄叶炎的妊娠母马出现高血压综合征的体征，胎盘血管存在血管异常，如微叶中血管管腔和毛细血管面积减小以及血管壁增厚。患慢性蹄叶炎母马所生的驹出生体重较低，妊娠期较短。
