Ueda Junko, Murata Yui, Bundo Miki, Oh-Nishi Arata, Kassai Hidetoshi, Ikegame Tempei, Zhao Zhilei, Jinde Seiichiro, Aiba Atsu, Suhara Tetsuya, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya
Laboratory for Molecular Dynamics of Mental Disorders, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako City, Saitama, 351-0198, Japan.
Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan; Department of Molecular Brain Science, Graduate School of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto City, Kumamoto, 860-8556, Japan.
Neurosci Res. 2017 Jul;120:60-65. doi: 10.1016/j.neures.2017.02.005. Epub 2017 Feb 17.
We examined the usefulness of commercially available DNA methylation arrays designed for the human genome (Illumina HumanMethylation450 and MethylationEPIC) for high-throughput epigenome analysis of the common marmoset, a nonhuman primate suitable for research on neuropsychiatric disorders. From among the probes on the methylation arrays, we selected those available for the common marmoset. DNA methylation data were obtained from genomic DNA extracted from the frontal cortex and blood samples of adult common marmosets as well as the frontal cortex of neonatal marmosets. About 10% of the probes on the arrays were estimated to be useful for DNA methylation assay in the common marmoset. Strong correlations existed between human and marmoset DNA methylation data. Illumina methylation arrays are useful for epigenome research using the common marmoset.
我们研究了用于人类基因组的市售DNA甲基化芯片（Illumina HumanMethylation450和MethylationEPIC）对普通狨猴（一种适用于神经精神疾病研究的非人灵长类动物）进行高通量表观基因组分析的实用性。在甲基化芯片的探针中，我们选择了可用于普通狨猴的那些探针。DNA甲基化数据来自成年普通狨猴额叶皮质和血液样本以及新生狨猴额叶皮质提取的基因组DNA。估计芯片上约10%的探针可用于普通狨猴的DNA甲基化检测。人类和狨猴的DNA甲基化数据之间存在很强的相关性。Illumina甲基化芯片可用于利用普通狨猴进行的表观基因组研究。
