Centre for Functional and Metabolic Mapping, Robarts Research Institute, and.
Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario N6A 5B7, Canada.
J Neurosci. 2019 Feb 6;39(6):1020-1029. doi: 10.1523/JNEUROSCI.2595-18.2018. Epub 2018 Dec 10.
The common marmoset () is a small New World primate species that has been recently targeted as a potentially powerful preclinical model of human prefrontal cortex dysfunction. Although the structural boundaries of frontal cortex were described in marmosets at the start of the 20th century (Brodmann, 1909) and refined more recently (Paxinos et al., 2012), the broad functional boundaries of marmoset frontal cortex have yet to be established. In this study, we sought to functionally derive boundaries of the marmoset lateral frontal cortex (LFC) using ultra-high field (9.4 T) resting-state functional magnetic resonance imaging (RS-fMRI). We collected RS-fMRI data in seven (four females, three males) lightly anesthetized marmosets and used a data-driven hierarchical clustering approach to derive subdivisions of the LFC based on intrinsic functional connectivity. We then conducted seed-based analyses to assess the functional connectivity between these clusters and the rest of the brain. The results demonstrated seven distinct functional clusters within the LFC. The functional connectivity patterns of these clusters with the rest of the brain were also found to be distinct and organized along a rostrocaudal gradient, consonant with those found in humans and macaques. Overall, these results support the view that marmosets are a promising preclinical modeling species for studying LFC dysfunction related to neuropsychiatric or neurodegenerative human brain diseases. The common marmoset is a New World primate that has garnered recent attention as a powerful complement to canonical Old World primate (e.g., macaques) and rodent models (e.g., rats, mice) for preclinical modeling of the human brain in healthy and diseased states. A critical step in the development of marmosets for such models is to characterize functional network topologies of frontal cortex in healthy, normally functioning marmosets, that is, how these circuitries are functionally divided and how those topologies compare to human circuitry. To our knowledge, this is the first study to demonstrate functional boundaries of the lateral frontal cortex and the corresponding network topologies in marmoset monkeys.
普通狨猴（Callithrix jacchus）是一种小型新世界灵长类动物，最近被作为人类前额叶皮层功能障碍的潜在强大临床前模型而受到关注。尽管 20 世纪初（Brodmann，1909）就已经描述了狨猴前额皮质的结构边界，并在最近进行了细化（Paxinos 等人，2012），但狨猴前额皮质的广泛功能边界尚未确定。在这项研究中，我们试图使用超高场（9.4 T）静息态功能磁共振成像（RS-fMRI）来从功能上推导狨猴外侧额皮质（LFC）的边界。我们在七只（四只雌性，三只雄性）轻度麻醉的狨猴中收集了 RS-fMRI 数据，并使用数据驱动的层次聚类方法，根据内在功能连接来推导 LFC 的细分。然后，我们进行了种子分析，以评估这些聚类与大脑其他部位之间的功能连接。结果表明，在 LFC 中有七个不同的功能聚类。这些聚类与大脑其他部位的功能连接模式也被发现是不同的，并沿着前后梯度组织，与人类和猕猴的发现一致。总体而言，这些结果支持了狨猴是研究与神经精神或神经退行性人类大脑疾病相关的 LFC 功能障碍的有前途的临床前建模物种的观点。普通狨猴是一种新世界灵长类动物，最近因其作为经典旧世界灵长类动物（例如猕猴）和啮齿动物模型（例如大鼠、小鼠）的有力补充，用于健康和患病状态下人类大脑的临床前建模而受到关注。在为这种模型开发狨猴的关键步骤是，在健康的、正常运作的狨猴中描述前额叶皮质的功能网络拓扑结构，即这些回路如何在功能上进行划分，以及这些拓扑结构如何与人类的回路结构进行比较。据我们所知，这是第一项证明狨猴外侧前额皮质和相应网络拓扑结构的功能边界的研究。
