Department of Molecular Biosciences and Bioengineering (MBBE), University of Hawai'i at Mānoa, 1955 East-West Road, Agricultural Science Building 218, Honolulu, HI 96822, USA.
College of Agriculture, Forestry and Natural Resource Management (CAFNRM), University of Hawai'i at Hilo, 200 W. Kawili Street, Hilo, HI 96720, USA.
Bioresour Technol. 2017 Aug;237:139-145. doi: 10.1016/j.biortech.2017.02.029. Epub 2017 Feb 10.
A series of batch experiments were conducted to investigate the effects of inoculum type, oxygen (O) dosage, and incubation time on volatile fatty acids (VFAs) production during anaerobic digestion (AD) of Napier grass (Pennisetum purpureum), a high yielding energy crop. The results showed that anaerobically digested cattle manure (ADCM) as an inoculum generated significantly higher VFAs compared to that of anaerobically digested waste activated sludge (ADWAS) as an inoculum. Additionally, the incubation time of 3days and O dosage of 15mL/g volatile solids showed the highest VFAs production when ADCM was used as an inoculum. Moreover, the VFAs production had a quadratic correlation with O dosage with R of 0.86. The Scanning Electron Microscopy (SEM) images of the digested fiber showed rough and crumbled surface structures as opposed to that of the undigested fiber, which was further confirmed by changes in structural composition of the digested fiber.
进行了一系列分批实验，以研究接种物类型、氧气 (O) 剂量和培养时间对高产生物量能源作物象草（Pennisetum purpureum）厌氧消化（AD）过程中挥发性脂肪酸（VFAs）生成的影响。结果表明，与作为接种物的厌氧消化废水活性污泥（ADWAS）相比，厌氧消化牛粪（ADCM）作为接种物产生的挥发性脂肪酸明显更高。此外，当 ADCM 用作接种物时，培养时间为 3 天，O 剂量为 15mL/g 挥发性固体时，挥发性脂肪酸的生成量最高。此外，VFAs 的生成与 O 剂量呈二次相关，相关系数为 0.86。消化纤维的扫描电子显微镜（SEM）图像显示，粗糙和碎裂的表面结构与未消化纤维相反，这进一步通过消化纤维结构组成的变化得到证实。
