Caballero-Gallardo Karina, Olivero-Verbel Jesus, Freeman Jennifer L
Environmental and Computational Chemistry Group. Campus of Zaragocilla. School of Pharmaceutical Sciences.University of Cartagena, Cartagena, Colombia.
School of Health Sciences. Purdue University, West Lafayette, IN 47907, USA.
Curr Genomics. 2016 Dec;17(6):515-527. doi: 10.2174/1389202917666160513105959.
The extent of our knowledge on the number of chemical compounds related to anthropogenic activities that can cause damage to the environment and to organisms is increasing. Endocrine disrupting chemicals (EDCs) are one group of potentially hazardous substances that include natural and synthetic chemicals and have the ability to mimic endogenous hormones, interfering with their biosynthesis, metabolism, and normal functions. Adverse effects associated with EDC exposure have been documented in aquatic biota and there is widespread interest in the characterization and understanding of their modes of action. Fish are considered one of the primary risk organisms for EDCs. Zebrafish (Danio rerio) are increasingly used as an animal model to study the effects of endocrine disruptors, due to their advantages compared to other model organisms. One approach to assess the toxicity of a compound is to identify those patterns of gene expression found in a tissue or organ exposed to particular classes of chemicals, through new technologies in genomics (toxicogenomics), such as microarrays or whole-genome sequencing. Application of these technologies permit the quantitative analysis of thousands of gene expression changes simultaneously in a single experiment and offer the opportunity to use transcript profiling as a tool to predict toxic outcomes of exposure to particular compounds. The application of toxicogenomic tools for identification of chemicals with endocrine disrupting capacity using the zebrafish model system is reviewed.
我们对与人为活动相关的、可能对环境和生物体造成损害的化合物数量的了解程度正在不断提高。内分泌干扰化学物质（EDCs）是一类潜在的有害物质，包括天然和合成化学物质，它们能够模拟内源性激素，干扰其生物合成、代谢和正常功能。与接触EDCs相关的不良影响已在水生生物群中得到记录，人们对其作用方式的表征和理解有着广泛的兴趣。鱼类被认为是EDCs的主要风险生物之一。斑马鱼（Danio rerio）由于与其他模式生物相比具有优势，越来越多地被用作研究内分泌干扰物影响的动物模型。评估化合物毒性的一种方法是通过基因组学（毒理基因组学）中的新技术，如微阵列或全基因组测序，识别在暴露于特定类别化学物质的组织或器官中发现的基因表达模式。这些技术的应用允许在单个实验中同时对数千个基因表达变化进行定量分析，并提供了将转录谱分析用作预测接触特定化合物毒性结果工具的机会。本文综述了利用斑马鱼模型系统应用毒理基因组学工具鉴定具有内分泌干扰能力的化学物质的情况。
