Landini M P, Severi B, Furlini G, Badiali De Giorgi L
Institute of Microbiology, Medical Faculty, University of Bologna, Italy.
Virus Res. 1987 Jul;8(1):15-23. doi: 10.1016/0168-1702(87)90036-0.
Using specific monoclonal antibodies, we have localized two structural proteins (p65-69 and p28) of human cytomegalovirus in infected cells and in virions and/or virus-related particles by immunoelectron microscopy using protein A-gold. Protein p65-69 is present in some roundish structures in the nuclei, often in contact with the viroplasm, and in the cytoplasm, exclusively within the dense body matrix. Protein p28 is present only in the outline of cytoplasmic capsids, and reaches the highest density in the large aggregates of virions and dense bodies which are particularly numerous during the late phases of the viral replication cycle.
利用特异性单克隆抗体，我们通过蛋白A-金免疫电子显微镜技术，在感染细胞以及病毒体和/或病毒相关颗粒中定位了人巨细胞病毒的两种结构蛋白（p65 - 69和p28）。蛋白p65 - 69存在于细胞核中的一些圆形结构中，常常与病毒质接触，并且存在于细胞质中，仅存在于致密体基质内。蛋白p28仅存在于细胞质衣壳的轮廓中，在病毒体和致密体的大聚集体中密度最高，而这些聚集体在病毒复制周期的后期特别多。
