人巨细胞病毒结构成分：p38的细胞内和病毒内定位

Human cytomegalovirus structural components: intracellular and intraviral localization of p38.

作者信息

Landini M P, Severi B, Cenacchi G, Lazzarotto T, Lindenmeier W, Necker A

机构信息

Institute of Microbiology, Faculty of Medicine, University of Bologna, Italy.

出版信息

Virus Res. 1991 May;19(2-3):189-98. doi: 10.1016/0168-1702(91)90045-w.

DOI:10.1016/0168-1702(91)90045-w

PMID:1654016

Abstract

Rabbit antisera raised against the product of ORF UL 80 of human cytomegalovirus (CMV) genome (HindIII L fragment of AD169 strain) as well as IgM from acutely infected patients recognize an antigen of Mr 38 kDa. In the viral particle this antigen is bound via S-S bridge to a lower Mr compound to form a final complex of 62 kDa that is also recognized by rabbit antisera as well as patients' IgM. P38 is present both in the nucleus and in the cytoplasm of CMV-infected cells starting from 24 h p.i. and increasingly thereafter. Immunoelectron microscopy revealed that this antigen is mainly associated with the internal portion of viral capsids both in the nucleus and in the cytoplasm.

摘要

针对人巨细胞病毒（CMV）基因组ORF UL 80产物（AD169株的HindIII L片段）制备的兔抗血清以及急性感染患者的IgM可识别一种分子量为38 kDa的抗原。在病毒颗粒中，该抗原通过二硫键与一种分子量较低的化合物结合，形成一种62 kDa的最终复合物，兔抗血清以及患者的IgM也可识别该复合物。从感染后24小时开始，P38存在于CMV感染细胞的细胞核和细胞质中，此后其含量不断增加。免疫电子显微镜显示，该抗原在细胞核和细胞质中主要与病毒衣壳的内部部分相关联。