Chen Stacey, Liu Geoffrey L, Li Marilyn M, Liu Renyu, Liu Henry
Department of Surgery, New York University Langone medical Center.
Division of Genomic Diagnostics, Department of Pathology & Laboratory Medicine, Children's Hospital of Philadelphia, Perelman School of Medicine at the University of Pennsylvania.
Transl Perioper Pain Med. 2017;2(1):13-19.
Epinephrine, a non-specific adrenergic agonist, is one of the most commonly used inotropes perioperatively. Recent studies have shown that inflammatory response in cardiac surgery could result in hypoperfusion, dysrhythmias, myocardial ischemia, and other pathophysiological alterations in the postoperative period. These alterations might be contributing to the adverse clinical outcome. Although epinephrine has been shown to have effects on the immune system, how epinephrine affects inflammatory response is unclear. We hypothesized that epinephrine exposure may alter the inflammatory response which may potentially contribute to the adverse clinical outcomes. We used cultured rat cardiomyocytes (H9C2) with epinephrine exposure in this study. The expression of mRNA for inflammation-related genes was quantitated for the comparison of experimental group (with epinephrine) and control group (without epinephrine). The results demonstrated significant changes of inflammation-related gene expressions in cardiomyocytes after epinephrine administration. The clinical implications of the gene expression changes in cardiomyocytes are unclear.
肾上腺素是一种非特异性肾上腺素能激动剂，是围手术期最常用的强心药之一。最近的研究表明，心脏手术中的炎症反应可能导致术后低灌注、心律失常、心肌缺血及其他病理生理改变。这些改变可能导致不良临床结局。虽然已证明肾上腺素对免疫系统有影响，但肾上腺素如何影响炎症反应尚不清楚。我们推测，暴露于肾上腺素可能会改变炎症反应，这可能会导致不良临床结局。在本研究中，我们使用了经肾上腺素处理的培养大鼠心肌细胞（H9C2）。对炎症相关基因的mRNA表达进行定量，以比较实验组（使用肾上腺素）和对照组（未使用肾上腺素）。结果表明，给予肾上腺素后心肌细胞中炎症相关基因表达发生了显著变化。心肌细胞中基因表达变化的临床意义尚不清楚。
