Zhang Jing-Jing, Wang Xin, Liu Dong
Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China.
Sheng Li Xue Bao. 2017 Feb 25;69(1):11-16.
This study aimed to investigate the expression of the natriuretic peptide precursor C coding gene nppc and its role in angiogenesis during embryonic period of the zebrafish. Whole mount in situ hybridization was performed to detect the expression pattern of nppc. nppc specific morpholino and nppc mRNA were injected respectively into the one-cell stage embryo to specifically knock-down and rescue the expression of nppc in Tg (flk1:GFP) and Tg (fli1a:nGFP) transgenic lines. The morphology and endothelial cell number of intersegmental vessel (ISV) were analyzed after imaging using the laser scanning confocal microscope. The results revealed that nppc was expressed in the brain, heart and vasculature of zebrafish larvae at 24 and 48 hours post-fertilization (hpf). Knock-down of nppc affected the development of ISV. Endothelial cell number was reduced after the knock-down of nppc. These results suggest that nppc controls zebrafish angiogenesis by affecting the endothelial cell proliferation and migration.
本研究旨在探讨利钠肽前体C编码基因nppc在斑马鱼胚胎期的表达及其在血管生成中的作用。采用整胚原位杂交技术检测nppc的表达模式。将nppc特异性吗啉代寡核苷酸和nppc mRNA分别注射到单细胞期胚胎中，以特异性敲低和挽救Tg(flk1:GFP)和Tg(fli1a:nGFP)转基因品系中nppc的表达。使用激光扫描共聚焦显微镜成像后，分析节间血管(ISV)的形态和内皮细胞数量。结果显示，nppc在受精后24和48小时(hpf)的斑马鱼幼体的脑、心脏和血管中表达。敲低nppc会影响ISV的发育。敲低nppc后内皮细胞数量减少。这些结果表明，nppc通过影响内皮细胞增殖和迁移来控制斑马鱼的血管生成。
