Chen Kan, Wang Chang-Qian, Fan Yu-Qi, Han Zhi-Hua, Wang Yue, Gao Lin, Zeng Hua-Su
Department of Cardiology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Sheng Li Xue Bao. 2017 Feb 25;69(1):55-60.
The present study aimed to study lipid-lowering effect of seven traditional Chinese medicine monomers in zebrafish system. Zebrafish were fed with high fat diet to establish a hyperlipemia model, then fasted and bathed with seven traditional Chinese medicine monomers stigmasterol, triacontanol, chrysophanol, vanillic acid, shikimic acid, polydatin and oleanolic acid respectively. The oil red O staining was used to detect the blood lipids of zebrafish. Serum total cholesterol and triglyceride levels were detected to validate the lipid-lowering effect. The result showed that a zebrafish model of hyperlipemia could be established by feeding larvae zebrafish with high fat diet. Among the seven traditional Chinese medicine monomers, chrysophanol had lipid-lowering effect. Chrysophanol significantly reduced serum total cholesterol and triglyceride levels in adult zebrafish fed with high fat diet. Chrysophanol accelerated peristalsis frequency of zebrafish intestine and the excretion of high fat food. It is concluded that chrysophanol has lipid- lowering effect in zebrafish, and the mechanism of the effect may be due to the roles of chrysophanol in reducing lipid absorption from gastrointestinal tract and accelerating the excretion of food.
本研究旨在探讨七种中药单体在斑马鱼体系中的降脂作用。用高脂饲料喂养斑马鱼以建立高脂血症模型，然后禁食并分别用七种中药单体豆甾醇、三十烷醇、大黄酚、香草酸、莽草酸、虎杖苷和齐墩果酸进行处理。采用油红O染色法检测斑马鱼的血脂。检测血清总胆固醇和甘油三酯水平以验证降脂效果。结果表明，用高脂饲料喂养斑马鱼幼鱼可建立高脂血症模型。在这七种中药单体中，大黄酚具有降脂作用。大黄酚显著降低了高脂饲料喂养的成年斑马鱼的血清总胆固醇和甘油三酯水平。大黄酚加快了斑马鱼肠道的蠕动频率以及高脂食物的排泄。结论是大黄酚在斑马鱼中有降脂作用，其作用机制可能是大黄酚在减少胃肠道脂质吸收和加速食物排泄方面发挥了作用。
