Research Centre for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin, China.
J Sep Sci. 2017 Apr;40(8):1694-1702. doi: 10.1002/jssc.201601372. Epub 2017 Mar 17.
In recent years, the efficient analysis of biological samples has become more important due to the advances of life science and pharmaceutical research and practice. Because biological sample pretreatment is the bottleneck for fast process, material development for efficient sample process in the high-performance liquid chromatography analysis is highly desirable. In this research, a cation-exchange restricted access monolithic column was synthesized by a reversible addition-fragmentation chain transfer polymerization method. Utilizing the controlled/living property of the reversible addition-fragmentation chain transfer method, a monolithic column of cross-linked poly(sulfopropyl methacrylate) was prepared first and then linear poly(glycerol mono-methacrylate) was immobilized covalently on the surface of the polymer. The monolithic material has both functionalities of cation-exchange and protein exclusion. Protein recovery of 94.6% was obtained after grafting of poly(glycerol mono-methacrylate) while the cation-exchange property of the column is still retained. In the study, the relation between the synthetic conditions and properties of the materials was studied. The synthesis conditions including the porogen, monomer concentration, and ratio of monomers/initiator/reversible addition-fragmentation chain transfer agent were optimized. The study provided a method for the preparation of restricted access monolithic columns: a bifunctional material by reversible addition-fragmentation chain transfer polymerization method.
近年来，由于生命科学和药物研究与实践的进步，对生物样品的高效分析变得更加重要。由于生物样品预处理是快速处理的瓶颈，因此在高效液相色谱分析中，开发用于高效样品处理的材料是非常需要的。在这项研究中，采用可逆加成-断裂链转移聚合方法合成了一种阳离子交换型受限流动整体柱。利用可逆加成-断裂链转移方法的可控/活性，首先制备交联聚（磺丙基甲基丙烯酰胺）整体柱，然后将线性聚（甘油单甲基丙烯酰胺）共价固定在聚合物表面。整体材料具有阳离子交换和蛋白质排斥两种功能。接枝聚（甘油单甲基丙烯酰胺）后，蛋白质回收率达到 94.6%，而柱的阳离子交换性能仍然保留。在研究中，研究了合成条件与材料性能之间的关系。优化了合成条件，包括致孔剂、单体浓度以及单体/引发剂/可逆加成-断裂链转移剂的比例。该研究提供了一种通过可逆加成-断裂链转移聚合方法制备受限流动整体柱的方法：一种双功能材料。
