College of Chemistry, Nankai University, Tianjin, P. R. China.
J Sep Sci. 2011 Jan;34(2):127-34. doi: 10.1002/jssc.201000255.
The "living"/controlled radical polymerization has provided an opportunity in making a more homogeneous polymer, which is favorable for polymer-based monolithic column fabrication. To study its application in the preparation of separation material, a capillary poly(ethylhexyl methacrylate-co-ethylene dimethacrylate) monolithic column has been synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. The correlation between the synthetic conditions and the polymer structures and separation performance was studied. The result indicated RAFT-mediated reaction provides condition for creating polymers with narrower pore size distribution and higher column efficiency compared with traditional polymerization. The "living" property of the RAFT polymerization was further utilized to graft hydrophilic polymer on the surface of poly(ethylhexyl methacrylate-co-ethylene dimethacrylate). The hydrophilic chain modified monolithic column has both abilities of protein exclusion and small hydrophobic compound retention. The result indicated that RAFT polymerization can be used for making multifunctional material. The restricted access monolithic material synthesized by this method can be used in biological sample analysis with HPLC direct injection.
“活性”/可控自由基聚合为制备更均匀的聚合物提供了机会，这有利于聚合物整体柱的制造。为了研究其在分离材料制备中的应用，通过可逆加成-断裂链转移（RAFT）聚合合成了毛细管聚（乙基己基甲基丙烯酸酯-共-乙二甲基丙烯酸酯）整体柱。研究了合成条件与聚合物结构和分离性能之间的关系。结果表明，与传统聚合相比，RAFT 介导的反应为创建具有更窄孔径分布和更高柱效的聚合物提供了条件。RAFT 聚合的“活性”性质进一步用于在聚（乙基己基甲基丙烯酸酯-共-乙二甲基丙烯酸酯）表面接枝亲水性聚合物。亲水性链修饰的整体柱同时具有蛋白质排斥和小分子疏水性化合物保留的能力。结果表明，RAFT 聚合可用于制备多功能材料。通过该方法合成的受限进入整体材料可用于 HPLC 直接进样的生物样品分析。
