Di Gregorio P, Rando A, Masina P
Istituto di Produzione Animale, Facoltà di Agraria, Università della Basilicata, Potenza, Italy.
Anim Genet. 1987;18(3):241-7. doi: 10.1111/j.1365-2052.1987.tb00764.x.
DNA samples obtained from sheep homozygous for HbA, for HbB and heterozygous were subjected to Southern blot analysis using a goat beta F and a rabbit beta 1-globin gene as probes. Sheep homozygous for HbA show a different restriction pattern from that of sheep homozygous for HbB with each of the used endonucleases. The DNAs from moufflon and sheep homozygous for HbA show indistinguishable restriction patterns with some endonucleases. By means of double digestions it has been possible to construct restriction maps of the beta B and beta C genes and to confirm the absence of the beta C gene in HbB sheep.
从纯合HbA、纯合HbB以及杂合的绵羊中获取DNA样本，使用山羊βF和兔β1 - 珠蛋白基因作为探针进行Southern印迹分析。对于每种使用的核酸内切酶，纯合HbA的绵羊显示出与纯合HbB的绵羊不同的限制性图谱。摩弗伦羊和纯合HbA的绵羊的DNA在某些核酸内切酶作用下显示出难以区分的限制性图谱。通过双酶切，得以构建βB和βC基因的限制性图谱，并证实HbB绵羊中不存在βC基因。
