Jin Chang Hyun, Park Han Chul, So Yangkang, Nam Bomi, Han Sung Nim, Kim Jin-Baek
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si, Jeollabuk-do 56212, Korea.
Department of Food and Nutrition, College of Human Ecology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Korea.
Molecules. 2017 Feb 17;22(2):311. doi: 10.3390/molecules22020311.
In this study, we aimed to compare supercritical carbon dioxide extraction and ethanol extraction for isoegomaketone (IK) content in perilla leaf extracts and to identify the optimal method. We measured the IK concentration using HPLC and inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells from the extracts. The IK concentration was 10-fold higher in perilla leaf extracts by supercritical carbon dioxide extraction (SFE) compared with that in perilla leaf extracts by ethanol extraction (EE). When the extracts were treated in LPS-induced RAW 264.7 cells at 25 μg/mL, the SFE inhibited the expression of inflammatory mediators such as nitric oxide (NO), monocyte chemoattractant protein-1 (MCP-1), interleutkin-6 (IL-6), interferon-β (IFN-β), and inducible nitric oxide synthase (iNOS) to a much greater extent compared with EE. Taken together, supercritical carbon dioxide extraction is considered the optimal process for obtaining high IK content and anti-inflammatory activities in leaf extracts from the P. frutescens Britt. radiation mutant.
在本研究中，我们旨在比较超临界二氧化碳萃取法和乙醇萃取法提取紫苏叶提取物中异欧前胡素（IK）的含量，并确定最佳方法。我们使用高效液相色谱法测定了提取物中IK的浓度，并在脂多糖（LPS）刺激的RAW 264.7细胞中检测了炎症介质。与乙醇萃取法（EE）提取的紫苏叶提取物相比，超临界二氧化碳萃取法（SFE）提取的紫苏叶提取物中IK浓度高10倍。当提取物以25μg/mL的浓度处理LPS诱导的RAW 264.7细胞时，与EE相比，SFE对炎症介质如一氧化氮（NO）、单核细胞趋化蛋白-1（MCP-1）、白细胞介素-6（IL-6）、干扰素-β（IFN-β）和诱导型一氧化氮合酶（iNOS）表达的抑制作用要强得多。综上所述，超临界二氧化碳萃取法被认为是从紫苏辐射突变体叶片提取物中获得高IK含量和抗炎活性的最佳工艺。
