内洛尔肉牛中与肉嫩度相关的基因多态性的基因型和等位基因频率。

Genotypic and allelic frequencies of gene polymorphisms associated with meat tenderness in Nellore beef cattle.

作者信息

Carvalho M E, Eler J P, Bonin M N, Rezende F M, Biase F H, Meirelles F V, Regitano L C A, Coutinho L L, Balieiro J C C, Ferraz J B S

机构信息

Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil

Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.

出版信息

Genet Mol Res. 2017 Feb 16;16(1):gmr-16-01-gmr.16018957. doi: 10.4238/gmr16018957.

DOI:10.4238/gmr16018957

PMID:28218779

Abstract

The objectives of this study were to characterize the allelic and genotypic frequencies of polymorphisms in the µ-calpain and calpastatin genes, and to assess their association with meat tenderness and animal growth in Nellore cattle. We evaluated 605 Nellore animals at 24 months of age, on average, at slaughter. The polymorphisms were determined for the molecular markers CAPN316, CAPN530, CAPN4751, CAPN4753, and UOGACAST1. Analyses of meat tenderness at 7, 14, and 21 days of maturation were performed in samples of longissimus thoracis obtained between the 12th and 13th rib and sheared using a Warner Bratzler Shear Force. Significant effects were observed for meat tenderness at days 7, 14, and 21 of maturation for the marker CAPN4751, at day 21 for the marker CAPN4753, and at days 14 and 21 for the marker UOGCAST1. For genotypic combinations of markers, the results were significant for the combination CAPN4751/UOGCAST1 in the three maturation periods and CAPN4753/UOGCAST1 at days 14 and 21 of maturation.

摘要

本研究的目的是确定μ-钙蛋白酶和钙蛋白酶抑制蛋白基因多态性的等位基因和基因型频率，并评估它们与内洛尔牛的肉嫩度和动物生长的关联。我们评估了平均24月龄屠宰时的605头内洛尔牛。确定了分子标记CAPN316、CAPN530、CAPN4751、CAPN4753和UOGACAST1的多态性。在第12和13肋骨之间获取的胸最长肌样本中，使用沃纳·布拉茨勒剪切力在成熟7、14和21天时对肉嫩度进行了分析。在成熟第7、14和21天时，标记CAPN4751对肉嫩度有显著影响；在第21天时，标记CAPN4753有显著影响；在成熟第14和21天时，标记UOGCAST1有显著影响。对于标记的基因型组合，在三个成熟时期，CAPN4751/UOGCAST1组合有显著结果；在成熟第14和21天时，CAPN4753/UOGCAST1组合有显著结果。