Pinto L F B, Ferraz J B S, Pedrosa V B, Eler J P, Meirelles F V, Bonin M N, Rezende F M, Carvalho M E, Cucco D C, Silva R C G
Departamento de Produção Animal, Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, Salvador, BA, Brasil.
Genet Mol Res. 2011 Sep 15;10(3):2057-64. doi: 10.4238/vol10-3gmr1263.
We analyzed single nucleotide polymorphisms in calpain, leptin, leptin receptor, and growth hormone receptor genes and their association with color, drip and cooking losses of longissimus muscle at 7, 14 and 21 days postmortem in 638 purebred Nellore bulls slaughtered between 22 and 26 months of age. Meat samples were vacuum-packed and aged at 4°C. The single nucleotide polymorphisms T945M, GHR2, E2FB, and CAPN4751 were evaluated. All genotypic classes were observed; however, the T/T genotype of T945M and E2FB was found at a low frequency. A significant association of E2FB with drip loss (a measure of water-holding capacity) was detected at seven days of meat aging. CAPN4751 had an additive effect on red and yellow color intensities. The T allele of CAPN4751 was found to be positively associated with improved meat color, but not with meat tenderness, differing from a previous report indicating that it is associated with meat tenderness. We conclude that the potential for use of CAPN4751 as a marker for these meat quality traits requires further research.
我们分析了钙蛋白酶、瘦素、瘦素受体和生长激素受体基因中的单核苷酸多态性，以及它们与638头22至26月龄屠宰的纯种内洛尔公牛宰后7天、14天和21天背最长肌的颜色、滴水损失和烹饪损失之间的关联。肉样真空包装后在4°C下陈化。评估了单核苷酸多态性T945M、GHR2、E2FB和CAPN4751。观察到了所有基因型类别；然而，T945M和E2FB的T/T基因型频率较低。在肉陈化7天时，检测到E2FB与滴水损失（一种持水能力的度量）之间存在显著关联。CAPN4751对红色和黄色强度有累加效应。发现CAPN4751的T等位基因与改善肉色呈正相关，但与肉的嫩度无关，这与之前报道其与肉的嫩度相关不同。我们得出结论，将CAPN4751用作这些肉质性状标记的潜力需要进一步研究。
