Department of Biomolecular Engineering and Genomics Institute, University of California, Santa Cruz, Santa Cruz, California, USA.
Nat Methods. 2017 Apr;14(4):411-413. doi: 10.1038/nmeth.4189. Epub 2017 Feb 20.
DNA chemical modifications regulate genomic function. We present a framework for mapping cytosine and adenosine methylation with the Oxford Nanopore Technologies MinION using this nanopore sequencer's ionic current signal. We map three cytosine variants and two adenine variants. The results show that our model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in Escherichia coli.
DNA 化学修饰调节基因组功能。我们提出了一种使用 Oxford Nanopore Technologies MinION 纳米孔测序仪的离子电流信号来绘制胞嘧啶和腺嘌呤甲基化的方法。我们绘制了三种胞嘧啶变体和两种腺嘌呤变体。结果表明，我们的模型足够灵敏，可以检测大肠杆菌生长阶段基因组 DNA 甲基化水平的变化。
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