Apoil P A, Puissant-Lubrano B, Congy-Jolivet N, Peres M, Tkaczuk J, Roubinet F, Blancher A
Laboratoire d'Immunogénétique Moléculaire, EA 3034, Université Paul Sabatier, Toulouse 3, France; Laboratoire d'Immunologie, CHU de Toulouse, France.
Laboratoire d'Immunologie, CHU de Toulouse, France.
Cell Immunol. 2017 Apr;314:42-53. doi: 10.1016/j.cellimm.2017.02.001. Epub 2017 Feb 9.
Using a standardized immunophenotyping procedure we studied thirty-eight distinct subpopulations of T, B and NK lymphocytes in 253 healthy blood donors aged from 19 to 67. We analysed the influence of age, sex and HCMV seropositivity on each lymphocyte subpopulations and established reference ranges. We observed that aging influences the largest number of lymphocyte subpopulations with a slow increase of CD8+ EMRA T lymphocytes and of the numbers of circulating Tregs. The proportion of HLA-DR+ cells among Tregs increased with age and was correlated to the proportion of HLA-DR+ cells among effector T CD4+ lymphocytes. Sex had a major impact on absolute counts of CD4+ T cells which were higher in females. HCMV-seropositivity was associated with higher frequencies of CD8+ EMRA memory T lymphocytes while a high frequency of terminally differentiated EMRA CD4+ T cells was observed in 80% of HCMV-positive individuals and in none of the HCMV seronegative individuals.
我们采用标准化免疫表型分析程序，对253名年龄在19至67岁的健康献血者的38个不同的T、B和NK淋巴细胞亚群进行了研究。我们分析了年龄、性别和人巨细胞病毒（HCMV）血清阳性对每个淋巴细胞亚群的影响，并建立了参考范围。我们观察到，衰老影响的淋巴细胞亚群数量最多，CD8 + EMRA T淋巴细胞数量和循环调节性T细胞（Tregs）数量缓慢增加。Tregs中HLA-DR + 细胞的比例随年龄增长而增加，并且与效应性CD4 + T淋巴细胞中HLA-DR + 细胞的比例相关。性别对CD4 + T细胞的绝对计数有重大影响，女性的CD4 + T细胞绝对计数更高。HCMV血清阳性与CD8 + EMRA记忆T淋巴细胞的较高频率相关，而在80%的HCMV阳性个体中观察到终末分化的EMRA CD4 + T细胞的高频率，而在HCMV血清阴性个体中未观察到。
