Naluyima Prossy, Eller Leigh Anne, Ouma Benson J, Kyabaggu Denis, Kataaha Peter, Guwatudde David, Kibuuka Hannah, Wabwire-Mangen Fred, Robb Merlin L, Michael Nelson L, de Souza Mark S, Sandberg Johan K, Eller Michael A
Makerere University Walter Reed Project, Kampala, Uganda.
Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden.
PLoS One. 2016 Jan 5;11(1):e0146196. doi: 10.1371/journal.pone.0146196. eCollection 2016.
Management of patient care and interpretation of research data require evaluation of laboratory results in the context of reference data from populations with known health status to adequately diagnose disease or make a physiological assessment. Few studies have addressed the diversity of lymphocyte subsets in rural and urban Ugandan populations. Here, 663 healthy blood bank donors from semi-urban centers of Kampala consented to participate in a study to define lymphocyte reference ranges. Whole blood immunophenotyping was performed to determine the frequency and absolute counts of T, B, and NK cells using clinical flow cytometry. Results from blood bank donors were compared to a rural cohort from the district of Kayunga and more urban clinical trial participants from the capital city, Kampala. Relationships between hematological and lymphocyte parameters were also explored. In the semi-urban blood donors, females were significantly different from males in all parameters except the frequency of CD8 T and B cells. Females had higher absolute counts of CD4 T, CD8 T and B cells, whereas males had higher NK cell counts. NK cell frequency and counts were significantly higher in semi-urban blood donors, regardless of sex, compared to more urban study participants. CD8 T cell frequency and counts were significantly higher in the blood donors compared to the rural participants, irrespective of sex. Interestingly, basophil counts were positively associated with overall T cell counts. These findings suggest that both sex and level of cohort urbanicity may influence lymphocyte subset distributions in Ugandans.
患者护理管理和研究数据解读需要在已知健康状况人群的参考数据背景下评估实验室结果，以便充分诊断疾病或进行生理评估。很少有研究探讨乌干达农村和城市人群中淋巴细胞亚群的多样性。在此，来自坎帕拉半城市中心的663名健康血库捐赠者同意参与一项研究，以确定淋巴细胞参考范围。使用临床流式细胞术进行全血免疫表型分析，以确定T细胞、B细胞和NK细胞的频率和绝对计数。将血库捐赠者的结果与来自卡永加区的农村队列以及来自首都坎帕拉的更多城市临床试验参与者的结果进行比较。还探讨了血液学和淋巴细胞参数之间的关系。在半城市血库捐赠者中，除CD8 T细胞和B细胞频率外，所有参数在女性和男性之间均存在显著差异。女性的CD4 T细胞、CD8 T细胞和B细胞绝对计数较高，而男性的NK细胞计数较高。与更多城市研究参与者相比，无论性别如何，半城市血库捐赠者的NK细胞频率和计数均显著更高。与农村参与者相比，无论性别如何，血库捐赠者的CD8 T细胞频率和计数均显著更高。有趣的是，嗜碱性粒细胞计数与总体T细胞计数呈正相关。这些发现表明，性别和队列城市化水平可能都会影响乌干达人的淋巴细胞亚群分布。
