Membrane Biochemistry & Biophysics, Bijvoet Center for Biomolecular Research and Institute of Biomembranes, Utrecht University, The Netherlands.
Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Aug;1862(8):797-799. doi: 10.1016/j.bbalip.2017.02.007. Epub 2017 Feb 17.
Mass spectrometry is increasingly used in research on membrane lipid homeostasis, both in analyses of the steady state lipidome at the level of molecular lipid species, and in pulse-chase approaches employing stable isotope-labeled lipid precursors addressing the dynamics of lipid metabolism. Here my experience with, and view on mass spectrometry-based lipid analysis is presented, with emphasis on aspects of quantification of membrane lipid composition of the yeast Saccharomyces cerevisiae. This article is part of a Special Issue entitled: BBALIP_Lipidomics Opinion Articles edited by Sepp Kohlwein.
质谱分析越来越多地应用于膜脂稳态的研究，既可以分析分子脂质种类水平的稳态脂组学，也可以采用稳定同位素标记的脂质前体进行脉冲追踪方法，研究脂质代谢的动力学。本文主要介绍了基于质谱的脂质分析的经验和观点，重点介绍了酵母酿酒酵母的膜脂组成定量方面。本文是由 Sepp Kohlwein 编辑的特刊“BBALIP_Lipidomics Opinion Articles”的一部分。
