Chen Yilu, Zhou Ling, Xu Jingjie, Zhang Lu, Li Mo, Xie Xingxing, Xie Yajuan, Luo Dan, Zhang Dalei, Yu Xiaochun, Yang Bei, Kuang Haibin
Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology and Department of Physiology, School of Medicine, Nanchang University, Nanchang, Jiangxi, China.
Reprod Toxicol. 2017 Apr;69:159-166. doi: 10.1016/j.reprotox.2017.02.010. Epub 2017 Feb 20.
Perfluorooctanoic acid (PFOA) is a synthetic perfluorinated compound, which has been reported to exert adverse effect on the pregnancy. However, whether it is associated with alteration of luteal function remains unknown. Mice were administered PFOA by gavage from gestational days (GD) 1-7 or 13. PFOA treatment did not significantly affect numbers of embryo implantation. Nevertheless, on GD 13, 10mg/kg PFOA treatment significantly increased numbers of resorbed embryo. Furthermore, PFOA exposure markedly reduced serum progesterone levels but did not affect estradiol levels. Treatment also showed concomitant decreases in transcript levels for key steroidogenic enzymes, and reduced numbers and sizes of corpora lutea. In addition, PFOA administration inhibited activities of superoxide dismutase and catalase, and increased generation of hydrogen peroxide and malondialdehyde, and down-regulated level of Bcl-2 and up-regulated p53 and BAX proteins. In conclusion, PFOA exposure significantly inhibits luteal function via oxidative stress and apoptosis in pregnant mice.
全氟辛酸（PFOA）是一种合成的全氟化合物，据报道其对妊娠有不良影响。然而，它是否与黄体功能改变有关仍不清楚。在妊娠第1 - 7天或第13天通过灌胃给小鼠施用PFOA。PFOA处理对胚胎着床数量没有显著影响。然而，在妊娠第13天，10mg/kg的PFOA处理显著增加了吸收胚胎的数量。此外，PFOA暴露显著降低了血清孕酮水平，但不影响雌二醇水平。处理还显示关键类固醇生成酶的转录水平同时降低，黄体的数量和大小减少。此外，施用PFOA抑制了超氧化物歧化酶和过氧化氢酶的活性，增加了过氧化氢和丙二醛的生成，并下调了Bcl-2水平，上调了p53和BAX蛋白。总之，PFOA暴露通过氧化应激和凋亡显著抑制妊娠小鼠的黄体功能。
