Veronesi Vanessa Barbosa, Batista Tatiane Helena, Ribeiro Ana Cláudia Alves Freire, Giusti-Paiva Alexandre, Vilela Fabiana Cardoso
Departamento de Ciências Fisiológicas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (Unifal-MG), Alfenas, Minas Gerais, Brazil; Programa de Pós-Graduação em Biociências Aplicadas à Saúde, Brazil.
Departamento de Ciências Fisiológicas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (Unifal-MG), Alfenas, Minas Gerais, Brazil; Programa de Pós-Graduação em Biociências Aplicadas à Saúde, Brazil.
Int J Dev Neurosci. 2017 May;58:74-81. doi: 10.1016/j.ijdevneu.2017.02.005. Epub 2017 Feb 20.
Dipyrone (metamizole), a powerful drug, is widely used as an analgesic and antipyretic; however, the safety of its use during lactation and the potential impact on the offspring are not well established. This study aimed to determine the effect of maternal dipyrone treatment during lactation on offspring development and emotional behavior and on the dam's maternal behavior. Hence, on postnatal day (PND) 2, drinking water only or drinking water containing dipyrone at doses of 100, 300, and 500mg/kg/day, were offered to lactating mothers up to PND9. Thereafter, all mice were provided regular drinking water. On PND2, all litters were culled to 8 pups (4 males and 4 females). Maternal behavior was evaluated at PND3, 6, 9, and 12, and at PND7 we evaluated locomotor activity in the open field. Reflex parameters and physical development of offspring were evaluated during lactation. At PND7, analysis of ultrasonic vocalization (USV) was performed. When the animals reached adolescence, we evaluated their performance in the open field, elevated plus maze (EPM), and marble burying. Our data demonstrated that maternal dipyrone treatment during lactation not only altered maternal behavior and the onset of physical and neurodevelopmental landmarks but also had an impact on anxiety-like behavior in offspring.
安乃近（甲氨基苯甲酸钠）是一种强效药物，被广泛用作镇痛药和解热药；然而，其在哺乳期使用的安全性以及对后代的潜在影响尚未明确。本研究旨在确定哺乳期母体安乃近治疗对后代发育、情绪行为以及母鼠母性行为的影响。因此，在出生后第2天（PND2），给哺乳期母鼠提供仅含饮用水或含剂量为100、300和500mg/kg/天安乃近的饮用水，直至PND9。此后，所有小鼠均提供常规饮用水。在PND2，将所有窝仔减少至8只幼崽（4只雄性和4只雌性）。在PND3、6、9和12评估母性行为，在PND7评估旷场中的运动活动。在哺乳期评估后代的反射参数和身体发育。在PND7进行超声发声（USV）分析。当动物进入青春期时，评估它们在旷场、高架十字迷宫（EPM）和埋大理石实验中的表现。我们的数据表明，哺乳期母体安乃近治疗不仅改变了母性行为以及身体和神经发育里程碑的出现时间，还对后代的焦虑样行为产生了影响。
