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聚合酶相关蛋白基因在新城疫病毒毒力中的作用

Roles of the Polymerase-Associated Protein Genes in Newcastle Disease Virus Virulence.

作者信息

Yu Xiao-Hui, Cheng Jin-Long, Xue Jia, Jin Ji-Hui, Song Yang, Zhao Jing, Zhang Guo-Zhong

机构信息

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University Beijing, China.

出版信息

Front Microbiol. 2017 Feb 6;8:161. doi: 10.3389/fmicb.2017.00161. eCollection 2017.

DOI:10.3389/fmicb.2017.00161
PMID:28220114
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5292418/
Abstract

The virulence of Newcastle disease virus varies greatly and is determined by multiple genetic factors. In this study, we systematically evaluated the roles of the polymerase-associated (NP, P and L) protein genes in genotype VII NDV virulence after confirming the envelope-associated (F and HN) proteins contributed greatly to NDV virulence. The results revealed that the polymerase-associated protein genes individually had certain effect on virulence, while transfer of these three genes in combination significantly affected the chimeric virus virulence, especially when the L gene was involved. These results indicated that the L protein was a major contributor to NDV virulence when combined with the homologous NP and P proteins. We also investigated viral RNA synthesis using NDV minigenome systems to assess the interaction between the NP, P, and L proteins, which showed that the activity of the polymerase-associated proteins were directly related to viral RNA transcription and replication.

摘要

新城疫病毒的毒力差异很大，由多种遗传因素决定。在本研究中，我们在确认包膜相关（F和HN）蛋白对新城疫病毒毒力有很大贡献后，系统评估了聚合酶相关（NP、P和L）蛋白基因在基因型VII新城疫病毒毒力中的作用。结果表明，聚合酶相关蛋白基因各自对毒力有一定影响，而这三个基因组合转移则显著影响嵌合病毒毒力，尤其是当L基因参与时。这些结果表明，当与同源NP和P蛋白结合时，L蛋白是新城疫病毒毒力的主要贡献者。我们还使用新城疫病毒微型基因组系统研究了病毒RNA合成，以评估NP、P和L蛋白之间的相互作用，结果表明聚合酶相关蛋白的活性与病毒RNA转录和复制直接相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/6bf036df39e5/fmicb-08-00161-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/7021459146ca/fmicb-08-00161-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/5a3a91c35577/fmicb-08-00161-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/8fa3e844ae30/fmicb-08-00161-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/36a7e55f2097/fmicb-08-00161-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/485e3ac5555f/fmicb-08-00161-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/6bf036df39e5/fmicb-08-00161-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/7021459146ca/fmicb-08-00161-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/5a3a91c35577/fmicb-08-00161-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/8fa3e844ae30/fmicb-08-00161-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/36a7e55f2097/fmicb-08-00161-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/485e3ac5555f/fmicb-08-00161-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9639/5292418/6bf036df39e5/fmicb-08-00161-g006.jpg

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