Goncharov N V, Sakharov I Iu, Danilov S M, Sakandelidze O G
Biull Eksp Biol Med. 1987 Sep;104(9):376-8.
The application of hepatopancreas collagenase from crab Paralithodes camtschatica for the isolation and cultivation of the endothelial cells was studied in human umbilical vein endothelium. The comparison of the enzyme from crab hepatopancreas with collagenase from Clostridium histoliticum has shown that the number of viable cells isolated from human umbilical vein by the crab enzyme was lower than in the case of microbial collagenase. However, this difference was not significant for subsequent cultivation of cells. Harvesting of the endothelial cells from the substrate during cultivation was more effective in the case when collagenase from crab hepatopancreas was used. It was shown that crab collagenase, in contrast with microbiological collagenase, was not a metal-dependent enzyme.
研究了堪察加拟石蟹肝胰腺胶原酶在人脐静脉内皮细胞中用于分离和培养内皮细胞的应用。将蟹肝胰腺酶与溶组织梭状芽孢杆菌胶原酶进行比较，结果表明，用蟹酶从人脐静脉分离出的活细胞数量低于微生物胶原酶的情况。然而，这种差异对细胞的后续培养并不显著。在培养过程中，使用蟹肝胰腺胶原酶时从底物收获内皮细胞更有效。结果表明，与微生物胶原酶相比，蟹胶原酶不是一种金属依赖性酶。
