Kang Yijun, Hao Yangyang, Xia Dan, Shen Min, Li Qing, Hu Jian
a Environmental Science & Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, People's Republic of China.
b College of Marine and Bio-engineering, Yancheng Teachers University, Yancheng, Jiangsu, People's Republic of China.
Can J Microbiol. 2017 Jul;63(7):563-572. doi: 10.1139/cjm-2016-0767. Epub 2017 Feb 21.
It is important to understand the dynamics of tetracycline-resistant bacteria (TRB) and tetracycline resistance genes (TRGs) in bulk and rhizosphere soils for evaluating the spread of TRGs from pig manure to human. In this work, a greenhouse experiment was conducted to investigate the difference in abundance of TRB, tetracycline-resistant Escherichia coli (TRE), tetracycline-resistant Pseudomonas spp. (TRP), and TRGs between bulk and cucumber rhizosphere soils. The application of pig manure resulted in the long-term persistence of TRB, TRE, TRP, and TRGs in bulk soil and rhizosphere of cucumber for at least 65 days. Pig manure application dose was the major driving force in altering the abundances of TRB and TRE, whereas TRP was disturbed mainly by compartment (bulk soil or rhizosphere). Both TRE and the percentage of TRE in bulk and rhizosphere soils increased linearly with an increase in dose of pig manure. The exponential relationships between pig manure dose and TRP along with TRP percentage were also noted. There were significant differences in the relative abundances of TRGs between bulk and cucumber rhizosphere soils, suggesting the use of pig manure exerted a more lasting impact on the spread of TRGs in the rhizosphere than in the bulk soil.
为评估四环素抗性基因从猪粪向人类的传播情况，了解四环素抗性细菌（TRB）和四环素抗性基因（TRG）在大块土壤和根际土壤中的动态变化非常重要。在这项研究中，进行了一项温室实验，以调查大块土壤和黄瓜根际土壤中TRB、四环素抗性大肠杆菌（TRE）、四环素抗性假单胞菌属（TRP）以及TRG丰度的差异。猪粪的施用导致TRB、TRE、TRP和TRG在大块土壤和黄瓜根际中长期存在至少65天。猪粪施用量是改变TRB和TRE丰度的主要驱动力，而TRP主要受土壤区域（大块土壤或根际）的影响。TRE以及大块土壤和根际土壤中TRE的百分比均随猪粪施用量的增加呈线性增加。还发现猪粪施用量与TRP以及TRP百分比之间呈指数关系。大块土壤和黄瓜根际土壤中TRG的相对丰度存在显著差异，这表明施用猪粪对TRG在根际中的传播产生的影响比在大块土壤中更持久。
