Barzi Samaneh Adami, Ghaderian Sayyed Mohammad Hossein, Noormohammadi Zahra
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Urogenital Stem Cell Research, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hum Antibodies. 2017;25(1-2):65-70. doi: 10.3233/HAB-170311.
Coronary artery disease (CAD) is the one of the most common heart diseases, being the main factor of mortality and morbidity worldwide. CAD has been known as a multifactorial disease and its progression depends on genetic and environmental factors. Numerous studies have shown evidence for association between 12q24.3 locus and CAD.
In the present study, the association of two HNF1A polymorphisms, rs2259816 and rs7310409, located on 12q24.3 locus with CAD was investigated in Iranian patients.
Whole genomic DNA was isolated from peripheral blood obtained from 411 Iranian volunteer. Then, the polymorphisms rs2259816 and rs7310409 located on 12q24.3 locus were genotyped using TaqMan Probe Real Time PCR. Statistical analysis was performed by SPSS software, version 19.
rs2259816 (p-value = 0.006) and rs7310409 (p-value = 0.001) showed statistically significant association with CAD risk. Our results indicated that there was a significant correlation between rs2259816 and blood triglyceride. However, no correlation was found between rs2259816, rs7310409 and other CAD risk factors.
冠状动脉疾病（CAD）是最常见的心脏病之一，是全球范围内死亡率和发病率的主要因素。CAD是一种多因素疾病，其进展取决于遗传和环境因素。大量研究表明12q24.3位点与CAD之间存在关联证据。
在本研究中，在伊朗患者中调查了位于12q24.3位点的两个HNF1A多态性rs2259816和rs7310409与CAD的关联。
从411名伊朗志愿者的外周血中分离全基因组DNA。然后，使用TaqMan探针实时PCR对位于12q24.3位点的多态性rs2259816和rs7310409进行基因分型。使用SPSS 19版软件进行统计分析。
rs2259816（p值 = 0.006）和rs7310409（p值 = 0.001）显示与CAD风险存在统计学显著关联。我们的结果表明rs2259816与血甘油三酯之间存在显著相关性。然而，未发现rs2259816、rs7310409与其他CAD风险因素之间存在相关性。
