Dickerson Vanna M, Rissi Daniel R, Brown Cathy A, Brown Scott A, Schmiedt Chad W
Departments of Small Animal Medicine, Surgery, College of Veterinary Medicine, University of Georgia, Athens, Georgia.
Athens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Georgia, Athens, Georgia.
Comp Med. 2017 Feb 1;67(1):56-66.
In an attempt to identify a feline model of acute or chronic kidney disease, this study was designed to evaluate the effects of 15 or 30 min of bilateral renal ischemia (RI) and 60 min of unilateral RI with delayed contralateral nephrectomy as models of acute kidney injury and chronic interstitial fibrosis in cats. Adult, purpose-bred, USDA Class A cats (n = 14) were randomly assigned to receive bilateral RI for 15 min (n = 3) or 30 min (n = 3), unilateral RI for 60 min with a delayed (2 wk) contralateral nephrectomy (n = 5), or sham unilateral RI with a delayed contralateral nephrectomy (n = 3). Serum creatinine concentration, urine specific gravity, and plasma clearance of iohexol were assessed at several time points throughout the study. Renal interstitial inflammatory cell counts and descriptive histopathology were acquired in all cats. Histomorphometry was used to quantify renal interstitial fibrosis and collagen at 120 d after RI in cats undergoing unilateral RI. Renal histopathology was evaluated at 21 and 120 d after bilateral and unilateral RI, respectively. Neither duration of bilateral RI resulted in appreciable histologic renal damage at 21 d after ischemia. At 120 d after ischemia, variable amounts of renal fibrosis were noted after 60 min of unilateral RI with delayed contralateral nephrectomy. Neither of the tested methods is a suitable model of consistent renal interstitial fibrosis in cats. Healthy cats appear able to sustain bilateral RI for as long as 30 min with no apparent effects on renal morphology or function at 21 d after ischemia.
为了确定急性或慢性肾病的猫模型，本研究旨在评估15或30分钟的双侧肾缺血（RI）以及60分钟的单侧RI并延迟对侧肾切除术作为猫急性肾损伤和慢性间质纤维化模型的效果。成年、专门繁育的美国农业部A类猫（n = 14）被随机分配接受15分钟（n = 3）或30分钟（n = 3）的双侧RI、60分钟的单侧RI并延迟（2周）对侧肾切除术（n = 5）或假单侧RI并延迟对侧肾切除术（n = 3）。在整个研究的多个时间点评估血清肌酐浓度、尿比重和碘海醇的血浆清除率。对所有猫进行肾间质炎性细胞计数和描述性组织病理学检查。对接受单侧RI的猫在RI后120天使用组织形态计量学来量化肾间质纤维化和胶原蛋白。分别在双侧和单侧RI后21天和120天评估肾脏组织病理学。双侧RI的持续时间在缺血后21天均未导致明显的肾脏组织学损伤。在缺血后120天，在60分钟的单侧RI并延迟对侧肾切除术后观察到不同程度的肾纤维化。所测试的两种方法都不是猫持续性肾间质纤维化的合适模型。健康的猫似乎能够耐受长达30分钟的双侧RI，在缺血后21天对肾脏形态或功能没有明显影响。
