Abdel-Moneim Ahmed S, Shehab Gaber M, Alsulaimani Adnan A, Al-Malky Mater I R
Microbiology Department, Virology Division, College of Medicine, Taif University, Al-Taif, 21944, Saudi Arabia; Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt.
Biochemistry Department, College of Medicine, Taif University, Al-Taif, 21944, Saudi Arabia; Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Mol Cell Probes. 2017 Jun;33:16-19. doi: 10.1016/j.mcp.2017.02.006. Epub 2017 Feb 20.
The human respiratory syncytial virus is a common respiratory pathogen in children. Improved diagnosis of the virus is dependent on the development of tools for the rapid detection and estimation of the viral loads. In the current study, RT-qPCR using TaqMan hydrolysis probe based on the F gene detection was developed to identify and quantify hRSV in clinical samples. The assay was validated by comparing the results with a commercially available RT-qPCR kit. The newly developed assay was sensitive in detecting hRSV positive samples (59/126) which were equivalent to those detected by the commercial kit (57/126) with a detection limit of 1 × 10 copies/mL. A high correlation was found between the results of the newly developed assay and the commercial one. It was concluded that the newly developed RT-qPCR assay can be used as a sensitive detection tool for hRSV-A.
人呼吸道合胞病毒是儿童常见的呼吸道病原体。病毒诊断的改善依赖于快速检测和估计病毒载量工具的开发。在本研究中，开发了基于F基因检测的TaqMan水解探针的RT-qPCR方法，以鉴定和定量临床样本中的人呼吸道合胞病毒。通过将结果与市售RT-qPCR试剂盒进行比较来验证该检测方法。新开发的检测方法在检测人呼吸道合胞病毒阳性样本（59/126）方面具有敏感性，与市售试剂盒检测的样本（57/126）相当，检测限为1×10拷贝/mL。新开发的检测方法与市售方法的结果之间发现高度相关。得出的结论是，新开发的RT-qPCR检测方法可作为人呼吸道合胞病毒A亚型的灵敏检测工具。
