Zhu Xinyan, Wang Shenglan, Shao Mingjie, Yan Jie, Liu Fei
Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
Department of Gastroenterology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200062, China.
Dev Comp Immunol. 2017 Jul;72:79-88. doi: 10.1016/j.dci.2017.02.007. Epub 2017 Feb 20.
Basigin (BSG), also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or cluster of differentiation 147 (CD147), plays various fundamental roles in the intercellular recognition involved in immunologic phenomena, differentiation, and development. In this study, we aimed to compare the similarities and differences of BSG among organisms and explore possible evolutionary relationships based on the comparison result. We used the extensive BLAST tool to search the metazoan genomes, N-glycosylation sites, the transmembrane region and other functional sites. We then identified BSG homologs from genomic sequences and analyzed their phylogenetic relationships. We identified that BSG genes exist not only in the vertebrate metazoans but also in the invertebrate metazoans such as Amphioxus B. floridae, D. melanogaster, A. mellifera, S. japonicum, C. gigas, and T. patagoniensis. After sequence analysis, we confirmed that only vertebrate metazoans and Cephalochordate (amphioxus B. floridae) have the classic structure (a signal peptide, two Ig-like domains (IgC2 and IgI), a transmembrane region, and an intracellular domain). The invertebrate metazoans (excluding amphioxus B. floridae) lack the N-terminal signal peptides and IgC2 domain. We then generated a phylogenetic tree, genome organization comparison, and chromosomal disposition analysis based on the biological information obtained from the NCBI and Ensembl databases. Finally, we established the possible evolutionary scenario of the BSG gene, which showed the restricted exon rearrangement that has occurred during evolution, forming the present-day BSG gene.
基底膜聚糖（BSG），也被称为细胞外基质金属蛋白酶诱导因子（EMMPRIN）或分化簇147（CD147），在涉及免疫现象、分化和发育的细胞间识别中发挥着多种重要作用。在本研究中，我们旨在比较不同生物中BSG的异同，并基于比较结果探索可能的进化关系。我们使用广泛的BLAST工具搜索后生动物基因组、N - 糖基化位点、跨膜区域和其他功能位点。然后，我们从基因组序列中鉴定出BSG同源物，并分析它们的系统发育关系。我们发现BSG基因不仅存在于脊椎动物后生动物中，也存在于无脊椎动物后生动物中，如佛罗里达文昌鱼、黑腹果蝇、意大利蜜蜂、日本血吸虫、太平洋牡蛎和巴塔哥尼亚锥虫。经过序列分析，我们证实只有脊椎动物后生动物和头索动物（佛罗里达文昌鱼）具有经典结构（一个信号肽、两个免疫球蛋白样结构域（IgC2和IgI）、一个跨膜区域和一个细胞内结构域）。无脊椎动物后生动物（不包括佛罗里达文昌鱼）缺乏N端信号肽和IgC2结构域。然后，我们根据从NCBI和Ensembl数据库获得的生物学信息生成了系统发育树、基因组组织比较和染色体定位分析。最后，我们建立了BSG基因可能存在的进化历程，其显示了在进化过程中发生的有限外显子重排，形成了现今的BSG基因。
