Chen Xiaoling, Luo Yanliu, Huang Zhiqing, Liu Guangmang, Zhao Hua
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China.
Oncotarget. 2017 Apr 11;8(15):25158-25166. doi: 10.18632/oncotarget.15374.
The objective of this study was to evaluate the effect of Akirin2 on slow myosin heavy chain (slow MyHC, MyHC I) gene expression and its molecular mechanisms. In this study, we showed that the protein expression of Akirin2 in pig slow oxidative Psoas major muscle is higher than that in fast glycolytic tibialis anterior muscle, suggesting that Akirin2 may play a role in myofiber typing. Knockdown of Akirin2 decreased the MyHC I expression and the calcineurin (CaN) activity, and also decreased the expressions of NFATc1 and MCIP1.4. Conversely, overexpression of Akirin2 got the opposite results. Furthermore, inhibition of CaN or knockdown of NFATc1 attenuated Akirin2 overexpression-induced upregulation of MyHC I. Together, these results demonstrate that Akirin2 promotes MyHC I expression via CaN/NFATc1 signaling pathway in porcine skeletal muscle satellite cells.
本研究的目的是评估Akirin2对慢肌球蛋白重链（慢肌球蛋白重链，肌球蛋白重链I）基因表达的影响及其分子机制。在本研究中，我们发现猪慢氧化型腰大肌中Akirin2的蛋白表达高于快糖酵解型胫骨前肌，这表明Akirin2可能在肌纤维类型划分中发挥作用。敲低Akirin2可降低肌球蛋白重链I的表达和钙调神经磷酸酶（CaN）的活性，同时也降低了NFATc1和MCIP1.4的表达。相反，过表达Akirin2则得到相反的结果。此外，抑制CaN或敲低NFATc1可减弱Akirin2过表达诱导的肌球蛋白重链I上调。总之，这些结果表明，Akirin2通过CaN/NFATc1信号通路促进猪骨骼肌卫星细胞中肌球蛋白重链I的表达。
