用于濒危龙脑香科植物的20个微卫星标记。
Twenty microsatellite markers for the endangered (Dipterocarpaceae).
作者信息
Guo Jun-Jie, Shang Shuai-Bin, Wang Chun-Sheng, Zhao Zhi-Gang, Zeng Jie
机构信息
Research Institute of Tropical Forestry, Chinese Academy of Forestry, Longdong, Guangzhou 510520, People's Republic of China.
Northwest Institute of Forest Inventory, Planning and Design, State Forestry Administration, Xi'an, Shaanxi 710048, People's Republic of China.
出版信息
Appl Plant Sci. 2017 Feb 6;5(2). doi: 10.3732/apps.1600134. eCollection 2017 Feb.
PREMISE OF THE STUDY
Microsatellite markers were developed for (Dipterocarpaceae), an endangered species indigenous to Southeast Asia and southern China.
METHODS AND RESULTS
Twenty microsatellite markers, including 12 polymorphic markers, were identified from using high-throughput sequencing. Polymorphism at each marker was evaluated using 87 individuals from three natural populations. The number of alleles per polymorphic locus ranged from six to 15, and the observed and expected heterozygosity varied from 0.000 to 0.926 and from 0.177 to 0.864, respectively. These markers were transferred successfully to the endangered species .
CONCLUSIONS
These markers may be used to investigate the genetic diversity and gene flow of and .
研究前提
为东南亚和中国南部本土的濒危物种（龙脑香科）开发了微卫星标记。
方法与结果
利用高通量测序从（该物种）中鉴定出20个微卫星标记，其中包括12个多态性标记。使用来自三个自然种群的87个个体评估每个标记的多态性。每个多态性位点的等位基因数量在6到15之间，观察到的杂合度和预期杂合度分别从0.000到0.926和从0.177到0.864不等。这些标记已成功转移到濒危物种（另一物种）。
结论
这些标记可用于研究（前一物种）和（后一物种）的遗传多样性和基因流。
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