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引用本文的文献

1
Isolation and characterization of twelve polymorphic microsatellite markers in the endangered (Dipterocarpaceae).濒危龙脑香科植物中12个多态性微卫星标记的分离与鉴定
Ecol Evol. 2020 Dec 2;11(1):4-10. doi: 10.1002/ece3.7077. eCollection 2021 Jan.
2
Exploring evolution and diversity of Chinese Dipterocarpaceae using next-generation sequencing.利用下一代测序技术探索中国龙脑香科的进化和多样性。
Sci Rep. 2019 Aug 12;9(1):11639. doi: 10.1038/s41598-019-48240-y.
3
Next Generation Sequencing-Based Molecular Marker Development: A Case Study in .基于新一代测序的分子标记开发：以. 为例
Molecules. 2018 Nov 13;23(11):2963. doi: 10.3390/molecules23112963.

用于濒危龙脑香科植物的20个微卫星标记。

Twenty microsatellite markers for the endangered (Dipterocarpaceae).

作者信息

Guo Jun-Jie, Shang Shuai-Bin, Wang Chun-Sheng, Zhao Zhi-Gang, Zeng Jie

机构信息

Research Institute of Tropical Forestry, Chinese Academy of Forestry, Longdong, Guangzhou 510520, People's Republic of China.

Northwest Institute of Forest Inventory, Planning and Design, State Forestry Administration, Xi'an, Shaanxi 710048, People's Republic of China.

出版信息

Appl Plant Sci. 2017 Feb 6;5(2). doi: 10.3732/apps.1600134. eCollection 2017 Feb.

DOI:10.3732/apps.1600134
PMID:28224060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5315383/
Abstract

PREMISE OF THE STUDY

Microsatellite markers were developed for (Dipterocarpaceae), an endangered species indigenous to Southeast Asia and southern China.

METHODS AND RESULTS

Twenty microsatellite markers, including 12 polymorphic markers, were identified from using high-throughput sequencing. Polymorphism at each marker was evaluated using 87 individuals from three natural populations. The number of alleles per polymorphic locus ranged from six to 15, and the observed and expected heterozygosity varied from 0.000 to 0.926 and from 0.177 to 0.864, respectively. These markers were transferred successfully to the endangered species .

CONCLUSIONS

These markers may be used to investigate the genetic diversity and gene flow of and .

摘要

研究前提

为东南亚和中国南部本土的濒危物种（龙脑香科）开发了微卫星标记。

方法与结果

利用高通量测序从（该物种）中鉴定出20个微卫星标记，其中包括12个多态性标记。使用来自三个自然种群的87个个体评估每个标记的多态性。每个多态性位点的等位基因数量在6到15之间，观察到的杂合度和预期杂合度分别从0.000到0.926和从0.177到0.864不等。这些标记已成功转移到濒危物种（另一物种）。

结论

这些标记可用于研究（前一物种）和（后一物种）的遗传多样性和基因流。