果蝇的金属硫蛋白基因。

The metallothionein gene of Drosophila.

作者信息

Maroni G, Otto E, Lastowski-Perry D, Price D H

机构信息

Department of Biology and Curriculum in Genetics, University of North Carolina, Chapel Hill 27514.

出版信息

Experientia Suppl. 1987;52:385-92. doi: 10.1007/978-3-0348-6784-9_36.

DOI:10.1007/978-3-0348-6784-9_36

PMID:2822463

Abstract

A chromosomal DNA segment containing the metallothionein gene was isolated from a genomic library of Drosophila melanogaster. A segment of 1543 bp was sequenced and found to include the structural sequence interrupted by one small intron. Within 300 bases upstream of the apparent site of transcription initiation, there are several short intervals very similar to the 12-base-pair segments considered to be responsible for metal regulation in mammalian systems. Several lines of evidence indicate that there is a single copy of the metallothionein gene (Mtn) in Drosophila. Mtn is located in the right arm of the third chromosome in region 85E10-15.

摘要

从黑腹果蝇的基因组文库中分离出了一段包含金属硫蛋白基因的染色体DNA片段。对一段1543 bp的片段进行了测序，发现其结构序列被一个小内含子打断。在转录起始位点上游300个碱基范围内，有几个短片段与被认为在哺乳动物系统中负责金属调控的12个碱基对片段非常相似。几条证据表明，果蝇中金属硫蛋白基因（Mtn）只有一个拷贝。Mtn位于第三染色体右臂的85E10 - 15区域。