Cho Eun Hye, Park Kyung Sun, Yang Mina, Song Dong Joon, Huh Hee Jae, Ki Chang Seok, Lee Nam Yong
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
SD Genomics Co., Ltd., Seoul, Korea.
Ann Lab Med. 2017 May;37(3):272-276. doi: 10.3343/alm.2017.37.3.272.
We describe the laboratory identification of Leptotrichia species from clinical isolates collected over a six-year period. Five isolates from blood cultures were identified as Leptotrichia species. Gram stain showed large, fusiform, gram-negative or -variable bacilli. Identification based on biochemical testing was unsuccessful; however, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved to be a useful tool for identifying Leptotrichia species to the genus level. Species level identification was successfully achieved by using 16S ribosomal RNA gene sequencing.
我们描述了从六年期间收集的临床分离株中对纤毛菌属菌种进行实验室鉴定的情况。从血培养物中分离出的五株菌株被鉴定为纤毛菌属菌种。革兰氏染色显示为大型、梭形、革兰氏阴性或革兰氏可变杆菌。基于生化测试的鉴定未成功；然而，基质辅助激光解吸/电离飞行时间质谱被证明是在属水平上鉴定纤毛菌属菌种的有用工具。通过使用16S核糖体RNA基因测序成功实现了种水平的鉴定。
