Arbefeville Sophie, Harris Anthony, Dittes Steven, Ferrieri Patricia
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School.
Lab Med. 2016 Aug;47(3):241-5. doi: 10.1093/labmed/lmw034. Epub 2016 Jul 4.
To identify the gram-negative rods grown from blood cultures and a right-knee fluid aspirate from an 80-year-old caucasian man who had undergone a total right knee arthroplastic procedure 6 years ago, and to assess the genetic similarity between the 2 isolates.
We used 3 different approaches: biochemical testing, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and 16S ribosomal RNA (rRNA) gene sequencing.
The 3 methods identified the gram-negative rods as Pasteurella multocida; 16S rRNA gene sequencing further identified the organisms as P. multocida subsp. septica.
A concordant identification of P. multocida was observed using biochemical testing, mass spectrometry, and 16S rRNA gene sequencing. Only 16S rRNA sequencing was able to determine the subspecies of P. multocida and to determine the genetic relatedness of the 2 isolates.
鉴定从血液培养物和一名80岁白人男性右膝液抽吸物中培养出的革兰氏阴性杆菌，该男性6年前接受了全右膝关节置换手术，并评估这两种分离株之间的基因相似性。
我们采用了3种不同的方法：生化检测、基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱分析和16S核糖体RNA（rRNA）基因测序。
这3种方法均将革兰氏阴性杆菌鉴定为多杀巴斯德菌；16S rRNA基因测序进一步将该菌鉴定为多杀巴斯德菌败血亚种。
使用生化检测、质谱分析和16S rRNA基因测序对多杀巴斯德菌的鉴定结果一致。只有16S rRNA测序能够确定多杀巴斯德菌的亚种，并确定这两种分离株的基因相关性。
