利用纳米颗粒和微磁体的快速免疫测定：以卵清蛋白模型进行概念验证
Rapid immunoassay exploiting nanoparticles and micromagnets: proof-of-concept using ovalbumin model.
作者信息
Delshadi Sarah, Blaire Guillaume, Kauffmann Paul, Fratzl Mario, Devillers Thibaut, Delabouglise Didier, Weidenhaupt Marianne, Dempsey Nora M, Cugat Orphée, Bruckert Franz, Marche Patrice N
机构信息
INSERM U1209, F-38000 Grenoble, France.
University Grenoble Alpes, IAB, F-38000 Grenoble, France.
出版信息
Bioanalysis. 2017 Mar;9(6):517-526. doi: 10.4155/bio-2016-0232. Epub 2017 Feb 22.
AIM
We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared with an ELISA. After optimization, the magnetic immunoassay exhibits a limit of detection (40 ng/ml) and a dynamic range (40-2500 ng/ml) similar to that of ELISAs developed using same biochemical tools.
CONCLUSION
Micromagnets can be fully integrated in multiwell plates at low cost to allow the efficient capture of immunocomplexes carried by magnetic nanoparticles. The method is generic and permits to perform magnetic ELISA in 30 min.
目的
我们展示了一种结合磁性纳米颗粒和微磁体的快速磁免疫测定法。利用微磁体产生的高磁场梯度开发了一种用于标准酶联免疫吸附测定（ELISA）的新方法。材料与方法/结果：基于比色法定量缓冲液中抗卵清蛋白抗体进行了概念验证，并与ELISA进行了比较。优化后，磁免疫测定法的检测限（40纳克/毫升）和动态范围（40 - 2500纳克/毫升）与使用相同生化工具开发的ELISA相似。
结论
微磁体可以低成本完全整合到多孔板中，以有效捕获磁性纳米颗粒携带的免疫复合物。该方法具有通用性，可在30分钟内完成磁ELISA检测。
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