Ito A, Krinsky N I, Cunningham M L, Peak M J
Division of Biological and Medical Research, Argonne National Laboratory, IL 60439-4833.
Free Radic Biol Med. 1987;3(2):111-8. doi: 10.1016/s0891-5849(87)80005-9.
Potassium superoxide (KO2) and xanthine-xanthine oxidase (X-XO), which are known generating systems for the superoxide anion, have different inactivating actions on Bacillus subtilis transforming DNA in vitro. Superoxide dismutase and CuSO4 enhanced the inactivation for KO2, but not for X-XO. Mannitol, a hydroxyl radical scavenger, protected against the inactivation by X-XO, but not by KO2. The results obtained with X-XO were consistent with the involvement of Fenton reactions, in which hydroxyl radical is the reactive species that ultimately causes damage. On the other hand, KO2-induced inactivation was partly due to the effect of H2O2. Differences in inactivation between the KO2 and X-XO systems may result from the different rates of production of the superoxide anion.
超氧化钾（KO₂）和黄嘌呤 - 黄嘌呤氧化酶（X - XO）是已知的超氧阴离子生成系统，它们对体外转化DNA的枯草芽孢杆菌具有不同的灭活作用。超氧化物歧化酶和硫酸铜增强了KO₂的灭活作用，但对X - XO没有作用。羟基自由基清除剂甘露醇可防止X - XO的灭活作用，但不能防止KO₂的灭活作用。用X - XO获得的结果与芬顿反应的参与一致，其中羟基自由基是最终导致损伤的活性物质。另一方面，KO₂诱导的灭活部分归因于H₂O₂的作用。KO₂和X - XO系统之间灭活作用的差异可能是由于超氧阴离子产生速率的不同。
