Guille J, Raison J K, Gebicki J M
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Free Radic Biol Med. 1987;3(2):147-52. doi: 10.1016/s0891-5849(87)80010-2.
The effect of radiation-induced peroxidation on the fluidity of the phospholipids of the erythrocyte membrane was studied using both erythrocyte ghosts and liposomes formed from the polar lipids of erythrocytes. In liposomes, the oxidation of the phospholipids increased with radiation dose, but there was no change in the fluidity of the lipids as measured by spin-label motion. Under the same conditions of irradiation, no oxidation of phospholipid was detected in erythrocyte ghosts, although changes occurred in the motion of spin labels intercalated with the membrane. These changes were attributed to radiation-induced alterations in the membrane proteins. It is concluded that alterations in motion of spin labels, observed with intact membranes after irradiation, are most likely the result of changes in the structure of membrane proteins rather than the lipids.
利用红细胞影和由红细胞极性脂质形成的脂质体，研究了辐射诱导的过氧化对红细胞膜磷脂流动性的影响。在脂质体中，磷脂的氧化随辐射剂量增加，但通过自旋标记运动测量的脂质流动性没有变化。在相同的辐照条件下，虽然嵌入膜中的自旋标记运动发生了变化，但在红细胞影中未检测到磷脂氧化。这些变化归因于辐射诱导的膜蛋白改变。得出的结论是，辐照后在完整膜中观察到的自旋标记运动变化很可能是膜蛋白结构变化而非脂质变化的结果。
