Kather H, Scheurer A
Klinisches Institut für Herzinfarktforschung, Heidelberg, Germany.
Horm Metab Res. 1987 Aug;19(8):379-81. doi: 10.1055/s-2007-1011829.
The effects of two phosphodiesterase inhibitors, e.g. theophylline and D-4-(3-butoxy-4 methoxybenzyl)-2-imidazolidinone (RO-20-1724) on the antilipolytic action of insulin in human adipocytes were investigated. At a concentration of 1 mmol/l (theophylline) and 0.1 mmol/l (RO-20-1724) both inhibitors increased cyclic AMP to a similar extent. However, their effects on insulin action were different. Whereas addition of theophylline abolished the ability of insulin to inhibit lipolysis, insulin was fully functional in depressing glycerol release in the presence of RO-20-1724. The results suggest that the insulin-sensitive phosphodiesterase of human adipocytes may be relatively resistant to RO-20-1724 as has been shown for the enzyme system of 3T3-L1 adipocytes.
研究了两种磷酸二酯酶抑制剂，即茶碱和D-4-（3-丁氧基-4-甲氧基苄基）-2-咪唑烷酮（RO-20-1724）对人脂肪细胞中胰岛素抗脂解作用的影响。在浓度为1 mmol/l（茶碱）和0.1 mmol/l（RO-20-1724）时，两种抑制剂使环磷酸腺苷（cAMP）升高的程度相似。然而，它们对胰岛素作用的影响不同。加入茶碱会消除胰岛素抑制脂解的能力，而在RO-20-1724存在的情况下，胰岛素在抑制甘油释放方面仍完全发挥作用。结果表明，人脂肪细胞中对胰岛素敏感的磷酸二酯酶可能像3T3-L1脂肪细胞的酶系统一样，对RO-20-1724相对不敏感。
